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Showing: 751 - 800 of 33753 results

  3. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid
    #214101
    Purpose
    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.
    Depositor
    Ting Zhou
    Article
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214101-plasmid-map-sequence-id-429357

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  8. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Expression
    Bacterial
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

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  9. pcDNA3HA-AR-LBD

    Plasmid
    #89111
    Purpose
    mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag
    Depositor
    Elizabeth Wilson
    Article
    He et al J Biol Chem. 1999 Dec 24;274(52):37219-2
    Insert
    hAR ligand binding domain (AR Human)
    Tags
    2xHA
    Expression
    Mammalian
    Mutation
    contains Ligand Binding Domain (624-919)
    Promoter
    CMV
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89111-plasmid-map-sequence-id-176143

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  23. HA-HA-AR-DBD-LBD

    Plasmid
    #171237
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171237-plasmid-map-sequence-id-336036

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  32. pCAG1.1-Galntl5 (37 kDa)-3xHA

    Plasmid
    #240646
    Purpose
    The nucleotides coding 37 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.
    Depositor
    Taichi Noda
    Article
    Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:
    Insert
    UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    240646-plasmid-map-sequence-id-484665

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  33. pCAG1.1-Galntl5 (30 kDa)-3xHA

    Plasmid
    #240647
    Purpose
    The nucleotides coding 30 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.
    Depositor
    Taichi Noda
    Article
    Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:
    Insert
    UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    240647-plasmid-map-sequence-id-484666

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  34. pCAG1.1-Galntl5 (20 kDa)-3xHA

    Plasmid
    #240648
    Purpose
    The nucleotides coding 20 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.
    Depositor
    Taichi Noda
    Article
    Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:
    Insert
    UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    240648-plasmid-map-sequence-id-484669

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  35. pCAG1.1-Galntl5 (10 kDa)-3xHA

    Plasmid
    #240649
    Purpose
    The nucleotides coding 10 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.
    Depositor
    Taichi Noda
    Article
    Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:
    Insert
    UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    240649-plasmid-map-sequence-id-484670

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  40. ρ1-EM GABAA receptor in pEZT-BM

    Plasmid
    #213072
    Purpose
    Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD
    Depositor
    Erik Lindahl
    Article
    Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.
    Insert
    Human GABAa rho1 receptor (GABRR1 Human)
    Use
    Bacmam, baculovirus, oocyte expression
    Tags
    Twin-Strep tag and superfolderGFP
    Expression
    Mammalian
    Mutation
    Encodes residues S58–L383 and D451–S479, separate…
    Promoter
    CMV and T7
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213072-plasmid-map-sequence-id-423917

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  43. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid
    #128584
    Purpose
    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128584-plasmid-map-sequence-id-254045

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  44. pLZRS-ER-Rac1-P29S-shortlinker

    Plasmid
    #128583
    Purpose
    For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Proline29 to Serine in the RAC1 moiety.
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128583-plasmid-map-sequence-id-253120

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  45. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

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  47. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178320-plasmid-map-sequence-id-363290

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  49. PGL4.11-COL1A1mSMAD

    Plasmid
    #230917
    Purpose
    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant potential SMAD bindin…
    Promoter
    COL1A1
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230917-plasmid-map-sequence-id-458138

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Showing: 751 - 800 of 33753 results