We narrowed to 59,251 results for: cloning

1. pICE LYS2-URA3

   Plasmid

   #122890

   Purpose

   LYS2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE ADE2-MET17

   Plasmid

   #122885

   Purpose

   ADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE LYS2-LEU2

   Plasmid

   #122888

   Purpose

   LYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICE TRP1-HIS3

   Plasmid

   #122891

   Purpose

   TRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE TRP1-MET17

   Plasmid

   #122893

   Purpose

   TRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFAP-Flex-MCS-WPRE-SV40 - GCH32

   Plasmid

   #230830

   Purpose

   AAV cloning vector for CRE-dependent expression under the GFAP promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15-MHL

   Plasmid

   #26092

   Purpose

   SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Sept. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Syn-FLEX-MCS-WPRE-SV40 - GCH8

   Plasmid

   #230818

   Purpose

   AAV cloning vector for CRE-dependent expression under the Syn promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. mOrange in pBSDONR P4r-P2

   Plasmid

   #121529

   Purpose

   For making C-terminal mOrange fusions using multisite Gateway cloning

   Depositor

   Roger Innes

   Insert

   mOrange
   

   Expression

   Bacterial

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. sCFP in pBSDONR P4r-P2

    Plasmid

    #121530

    Purpose

    For making C-terminal sCFP fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    sCFP
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-Flex-MCS-WPRE-SV40 - GCH26

    Plasmid

    #230827

    Purpose

    AAV cloning vector for CRE-dependent expression under the TRE promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaMKII-Flex-MCS-WPRE-SV40 - GCH20

    Plasmid

    #230824

    Purpose

    AAV cloning vector for CRE-dependent expression under the CaMKII promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME Actin Vhh mCherry (JDW 1240)

    Plasmid

    #224499

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mCherry
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-DreERT2 (JDW 1344)

    Plasmid

    #224523

    Purpose

    A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    DreERT2
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

    Plasmid

    #224536

    Purpose

    A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-FLAG- NLS-mScarlet-I3
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-3x-FLAG-Ubiquitin (JDW 1098)

    Plasmid

    #224513

    Purpose

    A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3x-FLAG-Ubiquitin
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME Actin-Vhh sfGFP (JDW 1215)

    Plasmid

    #224495

    Purpose

    Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME EFS-rtTA/rtTA3 (JDW 1230)

    Plasmid

    #224525

    Purpose

    A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS-rtTA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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