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AiP12514 - pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2514)

Plasmid

#191717

Purpose

AI plasmid ALIAS: AiP12514. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP11980 - pAAV-DLX2.0-minRho-dTomato-10aa-H2B-WPRE3-BGHpA (Alias: CN1980)

Plasmid

#229934

Purpose

DLX2.0 is an enhancer sequence, designed to drive nuclear dTomato expression in forebrain GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

dTomato-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11969 - pAAV-hsA2-AiE0140h-minRho-SYFP2-4X2C-WPRE-HGHpA (Alias: CN1969)

Plasmid

#223969

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12351 - pAAV-AiE0082h-minRho-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN2351)

Plasmid

#230208

Purpose

AiE0082h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12353 - pAAV-AiE0140h-minRho-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN2353)

Plasmid

#230210

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12352 - pAAV-AiE0128h-minRho-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN2352)

Plasmid

#230209

Purpose

AiE0128h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12350 - pAAV-AiE0079h-minRho-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN2350)

Plasmid

#230207

Purpose

AiE0079h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12343 - pAAV-AiE0354m-minRho-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN2343)

Plasmid

#230206

Purpose

AiE0354m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12342 - pAAV-AiE0354h-minRho-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN2342)

Plasmid

#230205

Purpose

AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14448: pAAV-3xSP10ins-AiE0367h-minRho-SYFP2-WPRE3-BarcodeA-BGHpA (Alias: CN4448)

Plasmid

#236587

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0367h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12293 - pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE3-BGHpA (Alias: CN2293)

Plasmid

#223980

Purpose

AiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12017 - pAAV-3xSP10ins-AiE0140h_3xC2-minRho-SYFP2-4X2C-WPRE3-BGHpA (Alias: CN2017)

Plasmid

#223970

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAIP4_2JHX

Plasmid

#234218

Purpose

Heterologous protein expression of rho GDP-dissociation inhibitor 1 (Rho GDI alpha) in Escherichia coli

Depositor

Christopher Bahl

Insert

2JHX (ARHGDIA Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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myc Rif wild-type

Plasmid

#38767

Depositor

Harry Mellor

Article

Pellegrin et al Curr Biol. 2005 Jan 26;15(2):129-33.

Insert

Rif (RHOF Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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myc Rif-QL

Plasmid

#38768

Depositor

Harry Mellor

Article

Pellegrin et al Curr Biol. 2005 Jan 26;15(2):129-33.

Insert

Rif (RHOF Human)

Tags

myc

Expression

Mammalian

Mutation

Q77L constitutively active point mutation

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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myc Rif-TN

Plasmid

#38769

Depositor

Harry Mellor

Article

Pellegrin et al Curr Biol. 2005 Jan 26;15(2):129-33.

Insert

Rif (RHOF Human)

Tags

myc

Expression

Mammalian

Mutation

T33N dominant-negative mutation

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-3xrGBD

Plasmid

#176091

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rnd1

Plasmid

#23227

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd1 (RND1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only