We narrowed to 17,057 results for: Ret;

1. pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

   Plasmid

   #209089

   Purpose

   mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM11-6xHis-TEV-hORF1p_R261A

   Plasmid

   #202586

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM11-6xHis-TEV-hORF1p_StammerAEA

   Plasmid

   #202588

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Methionine 91 to Alanine, changed O…

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRV∆L-5tTA

   Plasmid

   #182966

   Purpose

   The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   tTA
   

   Use

   Rabies

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX SYT9 C2A

   Plasmid

   #195707

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 124-211 of mouse SYT9

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX SYT9 C2B

   Plasmid

   #195708

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 253-344 of mouse SYT9

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX SYT9 C2AB

   Plasmid

   #195709

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 104-386 of mouse SYT9

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX SYT1 C2A

   Plasmid

   #195710

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 96-264 of mouse SYT1

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX SYT1 C2B

   Plasmid

   #195711

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 248-421 of mouse SYT1

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX SYT1 C2AB

    Plasmid

    #195712

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 96-421 of mouse SYT1

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET (SUMO)-Syt9

    Plasmid

    #195713

    Purpose

    Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Rat)
    

    Use

    Tags

    GST-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-RedO-shNC

    Plasmid

    #206356

    Purpose

    AAV plasmid expressing non-targeting control shRNA in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Non-targeting control shRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CMV-Txnip(C247S)

    Plasmid

    #206353

    Purpose

    AAV plasmid expressing mutant TXNIP

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    C247S

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE1176

    Plasmid

    #202774

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE1177

    Plasmid

    #202775

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCSh2-mRFP

    Plasmid

    #170982

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBMN BFP

    Plasmid

    #188644

    Purpose

    This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p5’UTR-ORF1

    Plasmid

    #190566

    Purpose

    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    

    Use

    For dna propagation only

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACT2-μ4

    Plasmid

    #198176

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-4 μ4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET30_Halotag_K73T_Jdomain

    Plasmid

    #183693

    Purpose

    Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)-J domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACT2-μ2

    Plasmid

    #198173

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-2 μ2
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

    Plasmid

    #185679

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-2xHaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UG75-PAH1-3HA

    Plasmid

    #184761

    Purpose

    PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PAH1 (PAH1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    PAH1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UG75-CDS1-3HA

    Plasmid

    #184760

    Purpose

    CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CDS1 (CDS1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    CDS1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. UG75-DGK1-3HA

    Plasmid

    #184759

    Purpose

    DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    DGK1 (DGK1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    DGK1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. OM14-2EGFP-UG74-ter

    Plasmid

    #184758

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    OM14 (OM14 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SEC7-2EGFP-ter-NAT

    Plasmid

    #184764

    Purpose

    Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    SEC7 (SEC7 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CIT1-2EGFP-ter-URA3

    Plasmid

    #184767

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CIT1 (CIT1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ACH1-2EGFP-ter-URA3

    Plasmid

    #184769

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    ACH1 (ACH1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge