We narrowed to 11,284 results for: Vars;

1. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   deletion of AA 1 to 45

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.4_CEACAM6-His

   Plasmid

   #149337

   Purpose

   for eukaryotic expression and purification of CEACAM6-His6

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Tags

   IL-2 signal peptide and polyhistidine

   Expression

   Mammalian

   Mutation

   point mutation (GGA to GCA) to replace 'G-g…

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

   Plasmid

   #139987

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2159

   Plasmid

   #70120

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-iCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Luc-T20

   Plasmid

   #177941

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2 (ORF1ab )
   

   Expression

   Mammalian

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-RNF168

   Plasmid

   #133976

   Purpose

   mammalian expression vector of Flag tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV-EF1a-ArgiNLS-AausFP1

   Plasmid

   #220595

   Purpose

   Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/Puro-CAG-ASAP2s

    Plasmid

    #101274

    Purpose

    Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

    Plasmid

    #221233

    Purpose

    Express sgRNA targeting HEK3 loci with nCas9(D10A)

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-P2A-EGFP
    

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159816

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

    Plasmid

    #61665

    Purpose

    Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
    

    Expression

    Mammalian

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1P POLA1_1

    Plasmid

    #160789

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe EGFR(L858R/T790M)

    Plasmid

    #32073

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L858R/T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TRKB-V5/HIS

    Plasmid

    #205614

    Purpose

    Expression of human TRKB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKB receptor tyrosine kinase (NTRK2 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe EGFR Del1

    Plasmid

    #32062

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Del1(E746-A750)

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. hWIP1 D314A FLAG

    Plasmid

    #28106

    Depositor

    Lawrence Donehower

    Article

    Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

    Insert

    WIP1 (PPM1D Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains phosphatase-dead mutation D314A

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUPD2 RDF ( GB1498)

    Plasmid

    #160579

    Purpose

    Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1-PDGFRB-V5/HIS

    Plasmid

    #201988

    Purpose

    expression of human PDGFRB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRB receptor tyrosine kinase (PDGFRB Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

    Plasmid

    #86674

    Purpose

    Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS E225A/D227A
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLVX-EF1a-tetOn-IRES-G418 (EtO)

    Plasmid

    #84776

    Purpose

    Second generation lenti tetracycline operator (on)

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    TET OPERATOR
    

    Use

    Lentiviral

    Promoter

    pTIGHT

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pKrox24(MapErk)Luc

    Plasmid

    #200112

    Purpose

    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence in minimal p…

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.1-TRKC-V5/HIS

    Plasmid

    #205615

    Purpose

    Expression of human TRKC receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKC receptor tyrosine kinase (NTRK3 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe EGFR Del1(T790M)

    Plasmid

    #32072

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Delete E746-A750, T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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