Addgene

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pFETCh_ZNF503

Plasmid

#135735

Purpose

Donor vector for 3' FLAG tag of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 Homology arms (ZNF503 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Use

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLBPres

Plasmid

#82454

Purpose

Encodes synthesized SLBP that is resistant to SMARTpool siRNA

Depositor

Bharat Ramratnam

Article

Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

Insert

SLBP (SLBP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
pPS1601

Plasmid

#8921

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

PAB1 (PAB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pPRP3 C3(1) promoter

Plasmid

#12490

Depositor

Jeffrey Green

Article

Shibata et al Cancer Gene Ther. 2001 Jan . 8(1):23-35.

Insert

C3(1) promoter (C3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCS2 xRor2-EGFP

Plasmid

#67163

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pClneoEGFP human RASSF6b

Plasmid

#37021

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF6b (RASSF6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
pLentiN 18E6no*

Plasmid

#37446

Purpose

Lentiviral expression of HPV 18E6no* in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 18E6no* (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

V44A

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pLNL1134

Plasmid

#160865

Purpose

PJ23110-RBS-ScEHT1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Medium-chain fatty acid ethyl ester synthase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Mutation

Promoter

PJ23110

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_NR2F6_2

Plasmid

#86343

Purpose

Encodes gRNA for 3' target of human NR2F6

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NR2F6 (NR2F6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_NR2F6_1

Plasmid

#86342

Purpose

Encodes gRNA for 3' target of human NR2F6

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NR2F6 (NR2F6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII Pig9

Plasmid

#16503

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig9 (MAD1L1 Human)

Use

Tags

Expression

Bacterial

Mutation

Partial cDNA clone

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pAGH23

Plasmid

#107262

Purpose

contains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

curT (slr0483 )

Use

Synthetic Biology

Tags

mTurquoise2

Expression

Bacterial

Mutation

Promoter

native CurT promoter

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG174

Plasmid

#105396

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

mVenus (GFP variant)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p6666 MSCV-IP N-HAonly 18E6

Plasmid

#44137

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV18 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus cerberus short

Plasmid

#24681

Depositor

Edward De Robertis

Article

Piccolo et al Nature. 1999 Feb 25. 397(6721):707-10.

Insert

cerberus (cer1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Cerberus short

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
3xDHFR-myc

Plasmid

#20216

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-RASSF1C

Plasmid

#1977

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

RASSF1C (Rassf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pJOG176

Plasmid

#105392

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

mEGFP (GFP variant)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXW101Plux2-gfp

Plasmid

#160819

Purpose

AHL-responsive single-layered circuit

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PJ23101, Plux2

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Flag-mPar6a.PIG

Plasmid

#24599

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
GFP-GW1D1a(aa254-503)

Plasmid

#21520

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503

Promoter

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SIN-PTEN-Y16C

Plasmid

#30372

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y16C (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

Y16C

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
NaVSp1 FL pIRES

Plasmid

#128075

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NaVAe1 FL pIRES

Plasmid

#128076

Purpose

Mammalian cell expression vector for NaVAe1 bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p6674 MSCV-IP N-HAonly 6bE6

Plasmid

#44139

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV6b E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
p6664 MSCV-IP N-HAonly 45E6

Plasmid

#44138

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV45 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
NHA-Ago4

Plasmid

#21537

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
pFETCh_ZNF331

Plasmid

#135726

Purpose

Donor vector for 3' FLAG tag of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 Homology arms (ZNF331 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZNF331_1

Plasmid

#135739

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZNF331_2

Plasmid

#135740

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-GW1D5(aa1670-1962)

Plasmid

#21519

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contain aa1670-1962

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
miR-vector plix4.03

Plasmid

#97141

Purpose

For lentiviral tet-inducible expression of miRNA and Dendra2

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Type

Empty backbone

Use

Lentiviral

Tags

dendra2

Expression

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opie2-PuroR[traF]

Plasmid

#131615

Purpose

Expresses a functional PuroR only in females and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Insert

PuroR[traF]

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_b4

Plasmid

#50698

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-GAP1IP4BPdeltaC2

Plasmid

#20199

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 19. ():.

Insert

GAP1IP4BP-detlaC2 (RASA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of C2 domains

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS311

Plasmid

#8903

Depositor

Pamela Silver

Article

Flach et al Mol Cell Biol. 1994 Dec . 14(12):8399-40

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
PE_3: Plux_lac_bD

Plasmid

#113604

Purpose

PE_3: Plux_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plux_lac_bD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Hybrid Promoter : Plux_lac_bD

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_13: Plux_lac_tet_bD

Plasmid

#113594

Purpose

PE_13: Plux_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plux_lac_tet_bD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Hybrid Promoter : Plux_lac_tet_bD

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_27: Plas_lac_tet_eG::sfYFP

Plasmid

#104888

Purpose

PE_27: Plas_lac_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Plas_lac_tet_eG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Hybrid Promoter: Plas_lac_tet_eG

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_28: Plux_lac_tet_eG::sfYFP

Plasmid

#104889

Purpose

PE_28: Plux_lac_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Plux_lac_tet_eG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plux_lac_tet_eG::sfYFP

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_29: Prhl_lac_tet_eG::sfYFP

Plasmid

#104890

Purpose

PE_29: Prhl_lac_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Prhl_lac_tet_eG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Hybrid Promoter : Pxyl_lac_tet::eG

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_30: Pxyl_lac_tet::eG:: sfYFP

Plasmid

#104891

Purpose

PE_30: Pxyl_lac_tet::eG:: sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Pxyl_lac_tet::eG::sfYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Hybrid Promoter : Pxyl_lac_tet::eG

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-CLCb

Plasmid

#47404

Depositor

Peter McPherson

Article

Legendre-Guillemin et al J Biol Chem. 2005 Feb 18;280(7):6101-8.

Insert

CLCb

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pBluescript Sp5

Plasmid

#16884

Depositor

Laure Bally-Cuif

Article

Tallafuss et al Development. 2001 Oct . 128(20):4021-34.

Insert

sp5 (sp5 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2008

Availability

Academic Institutions and Nonprofits only
pSuperRetro-puro-RalB 45i

Plasmid

#11122

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalAB siRNA (RALA, RALB Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pME-6MTzactMap2k2b

Plasmid

#67710

Purpose

Middle entry clone

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated Map2k2b (map2k2b Zebrafish)

Use

Tags

Expression

Mutation

S221D, S225D

Promoter

none

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Flag-mPar6aK19A

Plasmid

#24598

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19A

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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