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161,709 results

Showing: 4861 - 4890 of 161709 results

  2. SPYtag-Histag-Avidin

    Plasmid
    #214836
    Purpose
    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    SPYtag-Histag-Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214836-plasmid-map-sequence-id-423115

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  4. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  5. MIG-Luciferase-IRES-GFP

    Plasmid
    #75021
    Purpose
    Encodes the expression of Luciferase and GFP
    Depositor
    Saradhi Mallampati
    Insert
    Luciferase
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    75021-plasmid-map-sequence-id-337469

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  6. pKS diaCas9_sgRNA

    Plasmid
    #74923
    Purpose
    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter
    Depositor
    Per Winge
    Article
    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Cas9 module and sgRNA module
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74923-plasmid-map-sequence-id-149703

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  7. 6HisVDAC1/pCDNA3

    Plasmid
    #197320
    Purpose
    Plasmid expressing an antigen targeting the VDAC1 transporter
    Depositor
    Dan Minor
    Insert
    VDAC1 (VDAC1 Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    197320-plasmid-map-sequence-id-388161

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  9. pRS420

    Plasmid
    #11259
    Depositor
    Fred Cross
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11259-plasmid-map-sequence-id-6933

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  10. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  11. GFP-BAZ1A

    Plasmid
    #65371
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Article
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Insert
    BAZ1A (BAZ1A Human)
    Use
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    796I compared to all reference sequences
    Promoter
    CMV
    Available since
    June 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    208829-plasmid-map-sequence-id-419661

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  21. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  25. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Article
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  30. pCLX-PGK-hTERT

    Plasmid
    #114315
    Purpose
    Constitutive expression of the hTert protein
    Depositor
    Patrick Salmon
    Insert
    hTERT (TERT Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114315-plasmid-map-sequence-id-218852

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Showing: 4861 - 4890 of 161709 results