159,371 results
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pX-RCC1-HA
Plasmid#106938PurposeTransient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)DepositorInserthuman RCC1-HA (RCC1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3412
Plasmid#144888PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEGR1 (EGR1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-STAT3-S727D
Plasmid#73364PurposeExpression of STAT3-S727DDepositorInsertSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
UseTagsFLAG taggedExpressionMammalianMutationE16K S727DPromoterCMVAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV- κLight1.3
Plasmid#201223PurposeExpress kLight1.3 biosensor in mammalian cellsDepositorInsertκLight1.3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1wt
Plasmid#131810PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDG
Plasmid#187440PurposeVSV-G envelope expressing plasmidDepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLIK DN Rac1
Plasmid#84642PurposeLentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of VenusDepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationDominant NegativePromoterAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-P2A-mCherry BlastR
Plasmid#186743PurposeDox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertP2A-mcherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mApple-Sec61b-C1
Plasmid#90993PurposeExpresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmApple-Sec61b (SEC61B Human, Synthetic)
UseTagsmApple (red fluorescence)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLIX402 PHF2
Plasmid#91700Purposeinducible lentiviral expression of PHF2DepositorInsertPHF2 (PHF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
STIM1-EGFP
Plasmid#210370PurposeExpresses STIM1 fused with EGFP in mammalian cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CRBN-P2A-Hygro
Plasmid#124303PurposeLentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoterDepositorInsertCRBN (CRBN Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Control Vector
Plasmid#212937PurposeControl vector with constitutive firefly luciferase expressionDepositorInsertluc+
UseLuciferaseTagsExpressionMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR
Plasmid#182251PurposeHuman PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Talin-CS
Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast
Plasmid#205557PurposeExpress EPN24-PCP-IRES-eGFP and blasticidin resistance markerDepositorInsertEPN24-PCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible mCherry reporter
Plasmid#64128PurposePhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGW03 AAV-TBG-Cre-sgRNA
Plasmid#192163PurposeAAV-TBG-Cre-sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE4
Plasmid#90338PurposeNRF2 - Antioxidant response element gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNRF2Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet_N1
Plasmid#85054PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-45b(+)-Tn5
Plasmid#112112PurposeExpresses hyperactive Tn5 transposase for next generation sequencing library preparationDepositorInsertTn5
UseTagsHis6ExpressionBacterialMutationE54K E110K P242A L372PPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only
