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Showing: 6561 - 6600 of 171191 results

  9. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  25. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  31. LentiGuide Cyclone-Puro P2A EGFP

    Plasmid
    #247497
    Purpose
    Acyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion protein
    Depositor
    Samie Jaffrey
    Article
    Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4
    Insert
    puromycin n-acetyltransferase with Cyclone insertion
    Use
    Lentiviral and Synthetic Biology
    Tags
    Puro(Cyclone)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Cyclone was inserted after Glutamine135 of puromy…
    Promoter
    EF-1a
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    247497-plasmid-map-sequence-id-493033

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  34. pLV-SFFV-KTN1-WPRE-UbC-Emerald

    Plasmid
    #225941
    Purpose
    Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    KTN1 (KTN1 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225941-plasmid-map-sequence-id-446792

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Showing: 6561 - 6600 of 171191 results