We narrowed to 10,975 results for: NSI;

1. pAAV_hSyn1_AchLightG

   Plasmid

   #187464

   Purpose

   Expresses green acetylcholine indicator AchLightG in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   AchLightG
   

   Use

   AAV

   Tags

   Igκ leader

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLPC-FLAG.Mm.KAT5

   Plasmid

   #184647

   Purpose

   Retroviral expression of mouse Kat5

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   Kat5 (Kat5 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

   Plasmid

   #216763

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103_HCLS1-1/1

   Plasmid

   #91437

   Purpose

   Protein expression and purification of human SH3 domain construct HCLS1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   HCLS1-1/1 (HCLS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMVd1-EGFP-hRhoA-wt

   Plasmid

   #133926

   Purpose

   Low level expression of GFP fused human RhoA wild type

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   GFP-hRhoA WT (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. His-SUMO-Rad23A_pET11a

   Plasmid

   #169124

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hRad23A (RAD23A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-ArchT-tdTomato

   Plasmid

   #29778

   Purpose

   AAV-mediated expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-tdTomato
   

   Use

   AAV; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFBP_2xNbLYS1::LUZ

   Plasmid

   #189918

   Purpose

   Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

   Depositor

   Adam Steinbrenner

   Article

   Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Various

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pg-EGFP-HIF-2alpha

   Plasmid

   #87207

   Purpose

   N-terminally EGFP-tagged human HIF-2a/EPAS

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-2a (EPAS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q868E

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiCRISPRv2 Neo sgTREX1

    Plasmid

    #127645

    Purpose

    Knock-out of human TREX1 with NeoR

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    TREX1 sgRNA (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUDC175

    Plasmid

    #103019

    Purpose

    S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Use

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CamKII-ArchT-GFP (PV2527)

    Plasmid

    #99039

    Purpose

    AAV-mediated expression of ArchT under the CamKII promoter

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    ArchT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-E-cadherin promoter

    Plasmid

    #61798

    Purpose

    Luciferase reporter containing the mouse E-cadherin promoter

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pLJM1-TMEM192-FLAG

    Plasmid

    #164977

    Purpose

    Lentiviral vector for TMEM192 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLB038 - PHS1∆P

    Plasmid

    #136285

    Purpose

    entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    PHS1∆P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CaMKIV-dCT

    Plasmid

    #126422

    Purpose

    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-erExRai-AKAR2

    Plasmid

    #161756

    Purpose

    ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    CYP450-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX304_zeo

    Plasmid

    #160092

    Purpose

    Lentiviral vector for mammalian gene expression

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSW646 - pML1>>

    Plasmid

    #115995

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKD279.8

    Plasmid

    #173171

    Purpose

    Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Inserts

    SO_4388(REC)-PsdR(DBD) 137
    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23108 and PpsdA110

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pInducer20-Blast-CDT1-HA

    Plasmid

    #109335

    Purpose

    virus packaged, doxycyline inducible expression of Cdt1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cdt1 (CDT1 Human)
    

    Use

    Lentiviral; Inducible expression

    Tags

    HA, His, Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAS_4xMx PylT_FLAG-Mx PylRS YA

    Plasmid

    #140012

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A

    Promoter

    EF1

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-FLAG-MTOR-T1977R

    Plasmid

    #69011

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-T1977R (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Threonine 1977 to Arginine

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pDONR221_eGFP (open)

    Plasmid

    #160094

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAC_C_COX41

    Plasmid

    #111664

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    COX41 (COX4I1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSW648 - pREV>>

    Plasmid

    #115996

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiCRISPRv2 sgBLM10

    Plasmid

    #127643

    Purpose

    Knock-out of human BLM

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 sgRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP-N1-PHD2

    Plasmid

    #21401

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

33. NSK026 CMV-TO-p53R248W (FLP-IN)

    Plasmid

    #191168

    Purpose

    Expression of human p53 mutant R248W in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    p53(R248W) (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248W

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMTTH+mEGFP-H1.4

    Plasmid

    #157799

    Purpose

    Expression of mEGFP and human linker histone H1.4 fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-H1.4 (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

    Plasmid

    #192067

    Purpose

    Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for human FXOP2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid

    #101300

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-509, 670-698, and 699-906 …

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDEST40-2XHA-Src-Y419F

    Plasmid

    #140316

    Purpose

    Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. H2B-mAvicFP1

    Plasmid

    #129511

    Purpose

    Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    H2B-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 fused to the C terminus of human histone…

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

    Plasmid

    #100855

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    jRGECO1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge