We narrowed to 6,348 results for: Mag

1. pAAV-EF1a-DIO-FLPo-Myc

   Plasmid

   #124641

   Purpose

   AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   FLPo
   

   Use

   AAV and Cre/Lox

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-TO-RPB1-His K1268R CRres si2,4R

   Plasmid

   #139405

   Purpose

   Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K1268R

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG156 Mito-FAST

   Plasmid

   #130723

   Purpose

   Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   Mito-FAST
   

   Use

   Tags

   Myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG109 H2B-FAST

   Plasmid

   #130722

   Purpose

   Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   H2B-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-FDIO-ArchT-GFP

   Plasmid

   #124640

   Purpose

   AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   ArchT
   

   Use

   AAV; Flp/frt

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCrisprV2 sgRNA hPER2c-term #1

   Plasmid

   #179454

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCrisprV2 sgRNA hPER2c-term #2

   Plasmid

   #179455

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiCrisprV2 sgRNA hPER2c-term #3

   Plasmid

   #179456

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid

    #167573

    Purpose

    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Ribo-GCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-syn-jYCaMP1s

    Plasmid

    #135424

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRepair-mScI-CTNNB1

    Plasmid

    #153431

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCardinal-LaminB1-10

    Plasmid

    #56165

    Purpose

    Localization: Nuclear Envelope, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pDB-hCRY1-mClover3-CD4-bla

    Plasmid

    #179441

    Purpose

    Donor vector to knock in mClover3 C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mClover3
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid

    #202528

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. mCardinal-Actin-C-18

    Plasmid

    #56151

    Purpose

    Localization: Actin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    NM_001101.3 (ACTB Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

    Plasmid

    #63885

    Purpose

    An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized low-affinity GCaMP3(10.19)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBactin-EGFP-NEDD1-gTBD

    Plasmid

    #196857

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    EGFP-N-gTBD (NEDD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRepair-SGFP2-CTNNB1

    Plasmid

    #153430

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    SGFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFRT-TO-RPB1-His K1278R CRres si2,4R

    Plasmid

    #139406

    Purpose

    Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K1278R

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCardinal-MCAK-C-7

    Plasmid

    #56168

    Purpose

    Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    MCAK (KIF2C Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pX459-CTNNB1-ATG

    Plasmid

    #153429

    Purpose

    Expresses a gRNA that overlaps the startcodon of human CTNNB1

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 gRNA (CTNNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEX-YFP-STIM1-deltaK

    Plasmid

    #18861

    Depositor

    Tobias Meyer

    Article

    Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Stop codon inserted at amino acid postion 671 of …

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-puro/Flag PD-L1 4NQ

    Plasmid

    #121456

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDB-hCRY1-Halo-CD4-bla

    Plasmid

    #179445

    Purpose

    Donor vector to knock in Halotag C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    HaloTag (Halo7 Synthetic)
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-syn-FLEX-jYCaMP1

    Plasmid

    #135422

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Insert

    jYCaMP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-NDUFV2-mVenus

    Plasmid

    #185653

    Purpose

    NDUFV2 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-syn-FLEX-jYCaMP1s

    Plasmid

    #135420

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEX-CFP-STIM1-deltaK

    Plasmid

    #18860

    Depositor

    Tobias Meyer

    Article

    Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Stop codon at amino acid position 671 of STIM1. C…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge