We narrowed to 9,393 results for: Coli
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Plasmid#127350Purposegateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody (LaM-2)
Plasmid#162276PurposeExpresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.DepositorInsertmCherry-Nanobody (LaM-2)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-butelase1
Plasmid#115430PurposeExpresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coliDepositorInsertbutelase 1
UseTags6-HisExpressionMutationPromoterT5Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB578
Plasmid#141095PurposeE. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat arrayDepositorInsertsCas9 and tracrRNA
repeat-spacer-repeat array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDT-SuperFi-Cas9
Plasmid#183630PurposeExpression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coliDepositorInsertSuperFi-Cas9
UseCRISPRTags6xHis-MBPExpressionBacterialMutationY1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …PromoterT7Available sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPPac-hp
Plasmid#48123PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterDepositorInsertsignal peptide of Pac
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-HPF1-His-Sumo-Flag
Plasmid#111577PurposeExpresses full length HPF1 in E. coliDepositorInsertHPF1 (HPF1 Human)
UseTags6xHis-Sumo-FLAGExpressionBacterialMutationPromoterT7Available sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcI-ts,ind+-(mtSSB delta20)
Plasmid#186280PurposeExpresses Human Mitochondrial SSB in E. coliDepositorInsertMitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
UseTagsExpressionBacterialMutationFirst 20 amino acids corresponding to probable mi…PromoterlambdaAvailable sinceJuly 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
ThcCut1_pET29b
Plasmid#215429PurposeExpression construct for ThcCut1 gene, codon optimized for expression in E. coliDepositorInsertThcCut1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322-TIMER
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.7
Plasmid#78176PurposeExpresses rsGreen0.7 in E. coli strains.DepositorInsertrsGreen0.7
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-3_pET29b
Plasmid#215435PurposeExpression construct for UMG-SP-3 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NfPolyA_pET21b
Plasmid#215413PurposeExpression construct for NfPolyA gene, codon optimized for expression in E. coliDepositorInsertNfPolyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_A
Plasmid#168051PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSD057C
Plasmid#218136PurposeFor hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.DepositorInsertSD36
UseTagsrpoZExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDP004
Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-EsCas13d
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TR
Plasmid#89621Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG inductionDepositorInsertThioredoxin
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
FsC_pET29b
Plasmid#215405PurposeExpression construct for FsC gene, codon optimized for expression in E. coliDepositorInsertFsC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-CTCF(F1-F9)
Plasmid#102859PurposeExpress DNA binding domain of mouse CTCF in E. coliDepositorInsertCTCF (Ctcf Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d
Plasmid#108305PurposeExpresses E. coli codon optimized RspCas13d in the pET28a backbone.DepositorInsertRspCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP114
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
UseTagsExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneUseTagsDsbC-6His-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-AMSH*
Plasmid#66712PurposeExpresses activated AMSH in E. coliDepositorUseTagsHis-3CExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspWYL1
Plasmid#108306PurposeExpresses E. coli codon optimized RspWYL1 in the pET28a backbone.DepositorInsertRspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcI-ts,ind+-(NSP8)
Plasmid#160656PurposeExpresses codon optimized SARS-CoV-2 NSP8 in E. coliDepositorInsertSARS-CoV-2 NSP8 (ORF1ab Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only