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pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

Plasmid

#210016

Purpose

episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-Sox2-T2A-Klf4-E2A-Myc

Plasmid

#210018

Purpose

episomal SKM (expressing mouse Sox2 + Klf4 + Myc) for integration-free naïve reset

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry-t2a-SOX2-p2a-KLF4

Plasmid

#193296

Purpose

episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLNCX2-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90213

Purpose

To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

Insert

NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG-Flag-Sox9-IRES-nls-GFP

Plasmid

#44281

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

Flag-Sox9 (SOX9 Chicken)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV-IE enhancer and Chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

Plasmid

#169174

Purpose

piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175

Depositor

Igor Slukvin

Article

Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

Insert

SOX17-P2A-Venus (SOX17 Human, Synthetic)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

pTREtight (tet-inducible promoter)

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-Sox10-FLAG-tev-AVI

Plasmid

#127775

Purpose

Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox10 (SOX10 Chicken)

Use

Enhancer reporter construct

Tags

Avi

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCSC-ASCL1-IRES-GFP-T2A-Sox11

Plasmid

#182233

Purpose

To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

Depositor

Chun-Li Zhang

Article

Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

Insert

ASCL1-IRES-GFP-T2A-Sox11

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

Plasmid

#193359

Purpose

self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

T7

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

Plasmid

#163701

Purpose

Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Inserts

SOX2-3xHA-P2A-tagBFP (SOX2 Human)
rtTA
T2-sgRNA

Use

AAV and CRISPR

Tags

3x-HA and P2A-tagBFP

Expression

Mammalian

Mutation

Promoter

CAG and TRE3G

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-Sox17-p2A-Erg-t2A-GFP

Plasmid

#222496

Purpose

mouse fibroblast-to-endothelial cell direct reprogramming

Depositor

Li Qian

Article

Farber et al Nat Commun. 2024 May 16;15(1):4170. doi:

Inserts

Sox17 (Sox17 Mouse)
Erg (Erg )
EGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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SOX2-P2A-NLS-mClover3-IRES-Puro

Plasmid

#215544

Purpose

Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

Depositor

Kai Wang

Insert

SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

Plasmid

#195502

Purpose

Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2-p2a-KLF4

Plasmid

#193358

Purpose

VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4

Plasmid

#193295

Purpose

episomal vector expressing mCherry + human SOX2A61V + KLF4

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2AV-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Use

Tags

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

Plasmid

#210020

Purpose

tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO-mini-CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

Plasmid

#89991

Purpose

Donor template for generation of SOX2-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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