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Showing: 481 - 510 of 32526 results

  1. RP4-11

    Plasmid
    #79812
    Purpose
    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Kanamycin cassette and SacB gene (sacB )
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Km
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. pTJV1ScKm-rpoB

    Plasmid
    #166980
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    NeoR/KanR
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166980-plasmid-map-sequence-id-331333

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  5. pTC150

    Plasmid
    #70014
    Purpose
    'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Donor + GVR
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    70014-plasmid-map-sequence-id-124550

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  7. p006-Banana-Late

    Plasmid
    #112251
    Purpose
    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    ATF1 under osmY promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    osmY
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112251-plasmid-map-sequence-id-218818

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  12. pT2SK

    Plasmid
    #59383
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-kan
    Use
    Template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59383-plasmid-map-sequence-id-92127

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  13. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61272-plasmid-map-sequence-id-99585

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  18. pENTR-THT

    Plasmid
    #55790
    Purpose
    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Type
    Empty backbone
    Use
    RNAi; Gateway
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    modified H1 RNA gene
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55790-plasmid-map-sequence-id-89572

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  25. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid
    #73224
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL22-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL22
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73224-plasmid-map-sequence-id-135053

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  27. p005kanRFP

    Plasmid
    #58533
    Purpose
    RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58533-plasmid-map-sequence-id-90572

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Showing: 481 - 510 of 32526 results