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pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

Plasmid

#84474

Purpose

pAAV-RAM-NLS-mKate2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

mKate2

Use

AAV

Tags

Nuclear localization signal of SV40 large T antig…

Promoter

TRE

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

Plasmid

#71719

Purpose

Expressing a GFP-tagged degron in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

auxin-responsive protein IAA17 (AXR3 Mustard Weed)

Tags

EmGFP

Expression

Worm

Mutation

minimal degron sequence (71-114aa) with start cod…

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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ER-mRFP

Plasmid

#62236

Purpose

Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

Depositor

Erik Snapp

Article

Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

Insert

ER-mRFP

Tags

mRFP1

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUC18

Plasmid

#50004

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Type

Empty backbone

Expression

Bacterial

Promoter

lac

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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mIFP-N1

Plasmid

#54620

Purpose

Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Tags

mIFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hAtg2A

Plasmid

#36456

Depositor

Noboru Mizushima

Article

Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

Insert

autophagy-related protein 2 homolog A (ATG2A Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed Proline 656 to Arginine

Promoter

CMV

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO RasV12 Puro (w119-1)

Plasmid

#22262

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-CYLD

Plasmid

#22544

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-LKB1

Plasmid

#21147

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1 (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pBad-mKalama1

Plasmid

#14892

Purpose

Bacterial expression of mKalama1 blue fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

mKalama1

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pB18cmBFPAzurite

Plasmid

#14034

Depositor

Patrick Daugherty

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Insert

Azurite

Use

Yeast/bacteria optimized codons

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

Plasmid

#12153

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

6XHis

Expression

Mammalian

Available Since

July 7, 2006

Availability

Academic Institutions and Nonprofits only

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