164,577 results

1. pMyC

   Plasmid

   #42192

   Depositor

   Annabel Parret, Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   ColE1

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-tdTomato

   Plasmid

   #83029

   Purpose

   expresses tdTomato fluorescent protein under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST17-UbE2G1

   Plasmid

   #15790

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMSP2N2

   Plasmid

   #29520

   Depositor

   Stephen Sligar

   Article

   Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

   Insert

   MSP2N2
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   tandem MSP; His-tag on N-terminus followed by spa…

   Promoter

   Available Since

   May 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS578_DD-dSpyCas9-mCherry-APEX2

   Plasmid

   #108570

   Purpose

   Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Insert

   dSpyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   APEX2, Destabilization domains, Flag, and mCherry…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GRB2-V5

   Plasmid

   #139631

   Purpose

   V5-His-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GRB2 (GRB2 Human)
   

   Use

   Tags

   3xV5-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9n(BB)-2A-GFP (PX461)

   Plasmid

   #48140

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn1-FLEX-CybGam-WPRE

   Plasmid

   #207662

   Purpose

   Control for CybSEP2

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   Gamillus
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Histidine 86 and 159 to alanines, inserted gamill…

   Promoter

   hSynapsin

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcVpx.myc

    Plasmid

    #213388

    Purpose

    Generate lentiviral vector which increases gene delivery into primary human myeloid cells

    Depositor

    Nathaniel Landau

    Article

    Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

    Insert

    SIVmac vpx (vpx Simian Immunodeficiency Virus)
    

    Use

    Lentiviral

    Tags

    myc-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-KLC1

    Plasmid

    #166964

    Purpose

    Expresses Myc-tagged KLC1 protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1 (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1001

    Plasmid

    #143253

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SV-ABAactDA

    Plasmid

    #38247

    Depositor

    Jerry Crabtree

    Article

    Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

    Inserts

    VP16/PYL1
    Gal4DBD/ABI*
    

    Use

    Tags

    Flag, Gal4DBD, HA, and VP16

    Expression

    Mammalian

    Mutation

    D143A

    Promoter

    SV40 (IRES) and SV40p

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-6xGLY-His-tag-hphMX4

    Plasmid

    #20764

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid

    #174723

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and T2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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