161,873 results

1. pET28a-SpyTag002-MBP

   Plasmid

   #102831

   Purpose

   Bacterial expression of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to the N-terminus of MBP via a …

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197061

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS-21a his6-GST-WTPhi29DNAP

   Plasmid

   #166863

   Purpose

   To produce a dual affinity-tagged Phi29 DNA polymerase

   Depositor

   Stephen Quake, Paul Blainey

   Article

   Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

   Insert

   Phi29 Polymerase codon-optimized for overexpression in E. coli
   

   Use

   Tags

   GST and his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAA3.stelN

   Plasmid

   #112011

   Purpose

   Expresses TelN enzyme

   Depositor

   Stephen Harrison

   Article

   Jenni et al Science. 2018 May 4;360(6388):552-558. d

   Insert

   TelN
   

   Use

   Tags

   strep and his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-CAG-oScarlet-WPRE-BC6-pA

   Plasmid

   #220770

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   oScarlert-WPRE-BC6
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SlugCas9

   Plasmid

   #163793

   Purpose

   Expresses SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Rac1-2G

   Plasmid

   #66111

   Purpose

   Second generation Rac1 FRET biosensor for lentivirus production

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVoPo-GFP

   Plasmid

   #187840

   Purpose

   Constitutive GFP-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pToneDest

   Plasmid

   #67691

   Purpose

   attR4-attR3 Destination cassette in Tol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. phCMV-coGALVenv-C4070A

    Plasmid

    #172217

    Purpose

    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

    Depositor

    Boris Fehse

    Article

    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

    Insert

    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Carries C-terminus of amphotropic MLV strain 4070…

    Promoter

    CMV immediate early promoter

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSK659

    Plasmid

    #205128

    Purpose

    Expression of GFP-SnxA in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    SnxA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-mCherry

    Plasmid

    #180646

    Purpose

    Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-CMV-DsRed-IRES-d1EGFP

    Plasmid

    #41942

    Purpose

    Retroviral expression of DsRed in mammalian cells

    Depositor

    Stephen Elledge

    Article

    Yen et al Science. 2008 Nov 7;322(5903):918-23. do

    Insert

    DsRed
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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