19,634 results

1. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-puro-cMyc

   Plasmid

   #46970

   Purpose

   lentiviral expression of c-Myc

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-CMV-CD19 puro

   Plasmid

   #196634

   Purpose

   Express CD19

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-No-HaloTag7-mGFP-LC3

   Plasmid

   #184901

   Purpose

   Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-RFP

   Plasmid

   #22910

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   Chicken Beta-actin promoter driving DsRedExpress
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF0689 Ef1a-Pa01_attB-BFP acceptor

   Plasmid

   #193472

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RHCglo

   Plasmid

   #80169

   Purpose

   Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing

   Depositor

   Thomas Cooper

   Article

   Singh et al Biotechniques. 2006 Aug;41(2):177-81.

   Insert

   segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA4008

   Plasmid

   #70109

   Purpose

   mtDNA destruction/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   Herpes simplex virus UL12.5M185
   

   Tags

   none

   Expression

   Mammalian

   Mutation

   N-terminal deletion of UL12.5

   Promoter

   CMV and RSV

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    F74G

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEx-ER-TurboID

    Plasmid

    #160857

    Purpose

    Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Has Service

    AAV8

    Insert

    ER TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Promoter

    CAG

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG_WT MDA5

    Plasmid

    #216798

    Purpose

    Expresses wild type (wt) melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ERK-KTR-mStrawberry reporter

    Plasmid

    #158686

    Purpose

    This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_pMBP-EGFP-caax

    Plasmid

    #190155

    Purpose

    Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    EGFP-caax
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MBP

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-IGFBP5-V5

    Plasmid

    #11608

    Depositor

    Steven Johnson

    Insert

    Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-FLAG-SZT2

    Plasmid

    #87034

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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