163,606 results

1. Brg1 Del2 pET28a

   Plasmid

   #122116

   Purpose

   Expression Brg1 amino acids 1-837 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   6XHis and His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET3a-hUb

   Plasmid

   #61937

   Purpose

   Expression of hUb in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   human ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

3. HyPer7.2DAAO-mito

   Plasmid

   #168304

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-mito
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMY-mCherry-P2A-PuroR-HA

   Plasmid

   #163342

   Purpose

   Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

   Plasmid

   #129774

   Purpose

   Marker of microtubule dynamics in C. elegans hypodermis

   Depositor

   Andrew Chisholm

   Article

   Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

   Inserts

   Pcol-19
   ebp-2 (ebp-2 Nematode)
   unc-54 3'UTR
   

   Use

   Synthetic Biology; Gateway

   Tags

   Synthetic Intron and mGFP

   Expression

   Worm

   Mutation

   Promoter

   Pcol-19

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SF-U7snRNA-CypA-E4

   Plasmid

   #190695

   Purpose

   Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

   Depositor

   Pim Pijnappel

   Article

   van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

   Insert

   U7 snRNA antisense CypA Exon 4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U7 promoter

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Brg1 Del3 pET28a

   Plasmid

   #122117

   Purpose

   Expression Brg1 amino acids 836-1446 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRIS

   Plasmid

   #120424

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3, IS150
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid

    #176279

    Purpose

    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Terminase_PhiV1

    Plasmid

    #188836

    Purpose

    Large terminase from phage PhiV1

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Terminase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC6-NADK-3xFLAG

    Plasmid

    #163452

    Purpose

    lentiviral overexpression vector for human NADK

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    NADK (NADK Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    March 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-TMEM165

    Plasmid

    #132272

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    TMEM165 (TMEM165 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGEX Rin1-SH2

    Plasmid

    #46474

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Rin1 (RIN1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 54-204

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(-)-Green Falcan0.3

    Plasmid

    #199704

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan0.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(-)-Green Falcan3

    Plasmid

    #199706

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221_SLC9A8

    Plasmid

    #132199

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC9A8 (SLC9A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCIneo-lambdaN-HA-HsSmg6_I

    Plasmid

    #146551

    Purpose

    Mammalian Expression of HsSmg6

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsSmg6 (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T594C, A699G and two non sil…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TR-GFP

    Plasmid

    #80591

    Purpose

    Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available Since

    Sept. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJH4.66

    Plasmid

    #26951

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    beta-tubulin (tbb-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Hchk2 (kin-)-PUNI10

    Plasmid

    #41908

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    CHK2 (CHEK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    kinase dead (D347A)

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pLEX-EF1a ANXA11-mEmerald R346C

    Plasmid

    #164213

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R346C

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLEX-EF1a ANXA11-mEmerald R235Q

    Plasmid

    #164212

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R235Q

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pWT016i

    Plasmid

    #96856

    Purpose

    theophylline-agRNA-GFP

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    theophylline-agRNA-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221_SLC36A1

    Plasmid

    #132084

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC36A1 (SLC36A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. KHBD00297

    Plasmid

    #34423

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG10798 (Myc Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pPtPBR2

    Plasmid

    #80387

    Purpose

    Episome vector for Phaeodactylum tricornutum (pBR322-based)

    Depositor

    Philip Weyman

    Type

    Empty backbone

    Use

    Synthetic Biology; ; episomal vector for phaeodac…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GST-RNF126

    Plasmid

    #138643

    Purpose

    Bacterial expression of GST-RNF126 fusion protein

    Depositor

    Jane McGlade

    Article

    Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge