159,428 results
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Plasmid#172206PurposeExpresses KIF1A tagged with eGFP in mammalian cellsDepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Golgi
Plasmid#36205PurposeIn vivo visualization of golgi apparatus (can be used for colocalization studies)DepositorInsertGolgi targeting sequence (B4GALT1 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOS008: GCaMP6F calcium sensor (cytosolic)
Plasmid#163045PurposeGCaMP6F calcium sensor (cytosolic)DepositorInsertGCaMP6F
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-Linker-GFP
Plasmid#211734PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJMP3664
Plasmid#222350PurposeExpression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE57
Plasmid#90402PurposeType I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterGRAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FlagMLL-pl-ENL(5613)
Plasmid#20873DepositorUseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-EGFP
Plasmid#75159PurposeDerived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM3D(Gq)-mCherry
Plasmid#50478PurposeGq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM3D(Gq)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_MARV
Plasmid#135473PurposeMammalian expression vector encoding Marburg virus L gene under control of the AG promoterDepositorInsertL gene
UseTagsExpressionMammalianMutationsilent mutation (C to T) at position 7861; insert…PromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSDMA25
Plasmid#67940PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains nourseothricin resistance marker (NAT)DepositorTypeEmpty backboneUseCryptococcal complementation vectorTagsExpressionMutationPromoterACT1 for Cryptococcal markerAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_eGFP
Plasmid#26822DepositorInsert5'UTR-eGFP-3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRES2-hPAC
Plasmid#129488PurposeExpresses human TMEM206DepositorInsertTransmembrane protein 206 (PACC1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-LMNB1
Plasmid#207773PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
PLlacO-1-mfLon
Plasmid#45152DepositorInsertLon
UseSynthetic BiologyTagsssRA degradation (LVA)ExpressionBacterialMutationPromoterPLlacO-1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBL703
Plasmid#140373PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoterDepositorInsertT7-2BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL20C-ddIPpoI
Plasmid#49053PurposeExpresses the restriction enzyme IPpoI under the control of Shield1DepositorInsertDD-HA-ER-IPpoI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
PLEX-PGK-LAMP1-HaloTag
Plasmid#164216PurposepLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsHaloTagExpressionMutationPromoterPGKAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptet1
Plasmid#218360PurposeEmpty backbone with tetR-PtetA expression system with single tetR operator siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-EEA1
Plasmid#169067PurposeExpress human EEA1 in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-ARHGEF2
Plasmid#207959PurposeExpression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tagDepositorInsertARHGEF2 (ARHGEF2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUNIV backbone
Plasmid#24705DepositorTypeEmpty backboneUseXenopus oocyte expressionTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-3C-EmPNGaseF
Plasmid#218193PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseFDepositorInsertPNGaseF
UseTagstufA-His-CBD-3CExpressionBacterialMutationcodon optimized Ecoli, residues 41-354PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2538
Plasmid#144036PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX4 (SOX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF
Plasmid#131710PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-jGCaMP8m
Plasmid#213010PurposeTo study calcium signaling in AstrocytesDepositorInsertGfaABC1D-jGCaMP8m
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only