We narrowed to 17,014 results for: por

1. pCXLE-dCas9VP192-T2A-EGFP-shP53

   Plasmid

   #69535

   Purpose

   Episomal plasmid encoding dCas9VP192 and p53 shRNA

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-GFP-shp53
   

   Use

   CRISPR

   Tags

   EGFP

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-Fast-FT

   Plasmid

   #31913

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mRuby3

   Plasmid

   #171154

   Purpose

   Encodes mRuby3 fluorophore

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   mRuby3
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS1NB c Rosella I

   Plasmid

   #71245

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. P362-FBP_12

   Plasmid

   #139703

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKO2-N1

   Plasmid

   #54625

   Purpose

   Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

   Type

   Empty backbone

   Tags

   mKO2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTK_Citrine_Nanotag_24_positive_Control

   Plasmid

   #130570

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   FoxD3_cranial_neural_crest_enhancer_NC1
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE-Slow-FT

   Plasmid

   #31915

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Slow-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTXTL-T7max-FLuc

   Plasmid

   #188311

   Purpose

   Expression of Firefly luciferase via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   Firefly luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTight-hMYT1L-N174

    Plasmid

    #31877

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    myelin transcription factor 1-like (MYT1L Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Expression

    Mammalian

    Promoter

    U3MSCV

    Available Since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TadCBE-Cas9NG

    Plasmid

    #225093

    Purpose

    Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

    Insert

    TadCBE-Cas9NG
    

    Use

    CRISPR

    Tags

    8xHis

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUB-GFP

    Plasmid

    #11155

    Purpose

    Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    Ubiquitin C promoter (UBC Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMVRha

    Plasmid

    #229553

    Purpose

    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

    Depositor

    Sandy Schmidt

    Article

    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205550

    Purpose

    Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCCl-MCS_miniCMV_EGFP

    Plasmid

    #134984

    Purpose

    The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Promoter

    CAG

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

    Plasmid

    #132406

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKV016

    Plasmid

    #64767

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTK_Cerulean_Nanotag_70_neg_Control

    Plasmid

    #130568

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. P427-FBP_13

    Plasmid

    #139704

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205551

    Purpose

    Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP-I
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge