We narrowed to 17,014 results for: por

1. sgRNA 2 GAPDH

   Plasmid

   #83809

   Purpose

   Express Sg-2 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFast-FT-N1

   Plasmid

   #31910

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   CMV

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEP108E

   Plasmid

   #97394

   Purpose

   binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   sfGFP1-10OPT
   

   Tags

   Plasma membrane target

   Expression

   Plant

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV.hSyn.GLPLight1

   Plasmid

   #187466

   Purpose

   Expresses GLPLight1 in neurons

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight1
   

   Use

   AAV

   Tags

   Flag tag

   Promoter

   human Synapsin-1

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA 1 GAPDH

   Plasmid

   #83808

   Purpose

   Express Sg-1 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EpsilonCOP EGFP

   Plasmid

   #128151

   Purpose

   Expression of epsilon COP

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

   Insert

   epsilon COP (COPE Bovine)
   

   Expression

   Mammalian

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCMV Puro DEST P38KTRDronpa

   Plasmid

   #205763

   Purpose

   Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   P38 KTR Dronpa
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FRB-VMAc-CSarcin

   Plasmid

   #70223

   Purpose

   Expresses VMA intein with C-terminal portion of alpha-sarcin

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (C-terminal portion)
   

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Asp 28 to Cys, deleted amino acids 1-27

   Promoter

   CMV

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSplit2-NO-PRPF6

    Plasmid

    #51740

    Purpose

    Express LucN-PRPF6 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    PRP6 (PRPF6 Human)
    

    Tags

    FLAG and Luciferase (1-398aa)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. NSarQ27G-VMAn-FKBP

    Plasmid

    #70230

    Purpose

    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ3H PTP-1B

    Plasmid

    #8601

    Depositor

    Ben Neel

    Article

    Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

    Insert

    PTP-1B (PTPN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K103R

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mut1-RS

    Plasmid

    #182881

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut1-RS
    

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMedium-FT-N1

    Plasmid

    #31911

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a(+)::NL

    Plasmid

    #141289

    Purpose

    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDG04163

    Plasmid

    #216373

    Purpose

    xhNup214-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup214-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-scSUMO

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211622

    Purpose

    Rhno1 S141A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S141A

    Promoter

    CMV

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-GfaABC1D-GRAB_g5-HT3.0

    Plasmid

    #208727

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

    Plasmid

    #192636

    Purpose

    Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJAM1.21_PB-UGCG-PGK-Blast

    Plasmid

    #205566

    Purpose

    Express human UGCG

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    UGCG (UGCG Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid

    #202569

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AzoRS-4

    Plasmid

    #182884

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

    Insert

    AzoRS-4
    

    Expression

    Bacterial

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMPMA3dPlac-PpipB-GFPLVA

    Plasmid

    #23344

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Available Since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUPD+Red-F

    Plasmid

    #170874

    Purpose

    Phytobrick - Red-F Luciferase CDS

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    p.S286Y Red mutant

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2-epNTR

    Plasmid

    #62213

    Purpose

    epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

    Depositor

    Harold Burgess

    Article

    Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

    Insert

    epNTR
    

    Use

    Mrna synthesis

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAL101

    Plasmid

    #22474

    Purpose

    Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    No promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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