We narrowed to 15,050 results for: pET

1. pJMP1050

   Plasmid

   #119240

   Purpose

   construction intermediate

   Depositor

   Jason Peters, Carol Gross, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 2Bc-T MBP_BoMoC(ed)_6xH

   Plasmid

   #186461

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NSUN2

   Plasmid

   #188059

   Purpose

   Bacterial expression plasmid for human NSUN2; for activity testing

   Depositor

   Tanja Schirmeister

   Article

   Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

   Insert

   NSUN2 (NSUN2 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OPEN-A*24:02

   Plasmid

   #235133

   Purpose

   E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*24:02 (HLA-A Human)
   

   Expression

   Bacterial

   Mutation

   G120C

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OPEN-HLA-A*01:01

   Plasmid

   #235134

   Purpose

   E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*01:01 (HLA-A Human)
   

   Expression

   Bacterial

   Mutation

   G120C

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 6His-MBP-TEV-huAsCpf1

   Plasmid

   #90095

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huAsCpf1
   

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 6His-MBP-TEV-huLbCpf1

   Plasmid

   #90096

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GB1-A11(2-196, G38R)-Strep

    Plasmid

    #196209

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G38R

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB1-A11(2-196, G175R)-Strep

    Plasmid

    #196211

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G175R

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LIC1B_Caspase-LOVN7C-7

    Plasmid

    #104629

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Tags

    His and LOV2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 6xHis-MBP-BrCas12b

    Plasmid

    #182276

    Purpose

    Expresses recombinant BrCas12b

    Depositor

    Piyush Jain

    Article

    Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, T7, Thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEAT8-137 M1V

    Plasmid

    #173807

    Purpose

    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

    Depositor

    Anne Gershenson

    Article

    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

    Insert

    SERPiNA1 (SERPINA1 Human)
    

    Expression

    Bacterial

    Mutation

    E1M Otherwise this plasmid encodes the most commo…

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His10-CD3z-SNAPf-ybbR (XSB507)

    Plasmid

    #78508

    Purpose

    Construct for expressing CD3z-SNAPf-ybbR in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD3z (Cytoplasmic domain) (CD247 Human)
    

    Tags

    His10 and SNAPf-ybbR

    Expression

    Bacterial

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NSUN6

    Plasmid

    #188060

    Purpose

    Bacterial expression plasmid for human NSUN6; for activity testing

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

    Insert

    NSUN6 (NSUN6 Human)
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-GIV-CTs

    Plasmid

    #69864

    Purpose

    Express His tagged GIV-CTs (1660–1870).

    Depositor

    Pradipta Ghosh

    Article

    Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C terminal amino acids S1660-S1870 of human GIV.

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FAK2_HUMAN_D0

    Plasmid

    #79747

    Purpose

    This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FAK2 (PTK2B Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

22. STK24_HUMAN_D0

    Plasmid

    #79742

    Purpose

    This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK24 (STK24 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

23. ZIKV_Trigger_27B

    Plasmid

    #75008

    Purpose

    Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_27B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MEF2A-Sensor

    Plasmid

    #208928

    Purpose

    in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    Mef2a docking sequence+Phospho site (MEF2A Human)
    

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SLK_HUMAN_D0

    Plasmid

    #79733

    Purpose

    This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SLK (SLK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

26. ZIKV_Trigger_32B

    Plasmid

    #75009

    Purpose

    Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_32B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CDK2_HUMAN_D0

    Plasmid

    #79726

    Purpose

    This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDK2 (CDK2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

28. DYRK2_HUMAN_D0

    Plasmid

    #79729

    Purpose

    This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK2 (DYRK2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

29. ZIKV_NASBA_32B

    Plasmid

    #75011

    Purpose

    Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_32B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. STK16_HUMAN_D0

    Plasmid

    #79701

    Purpose

    This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK16 (STK16 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only