20,120 results
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HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
mAmetrine-C1
Plasmid#54660PurposeLocalization: C1 Cloning Vector, Excitation: 406, Emission: 526DepositorTypeEmpty backboneTagsmAmetrineExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
hRad51(A89E)–Cas9(D10A)
Plasmid#125563PurposeExpresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-OptoFGFR1
Plasmid#59776PurposeExpresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused proteinDepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX
Plasmid#167582PurposeExpress the ATP sensor GRAB_ATP1.0 in mammalian cellsDepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xM67 pTATA TK-Luc
Plasmid#8688DepositorInsert4 repeated M67 sites
UseLuciferaseExpressionMammalianAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Cherry
Plasmid#170510PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
mCherry
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB506
Plasmid#74848PurposeGateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2
Plasmid#221225PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporterDepositorInsertAID-PcrA M6-UGI-T2A-mTagBFP2
ExpressionMammalianAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 WT
Plasmid#16240DepositorAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL4A-pCMV7.1
Plasmid#155022PurposeN-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vectorDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Basic-Myogenin core promoter
Plasmid#134722PurposeMyogenin core promoter in a luciferase reporter plasmidDepositorInsertMyogenin Promoter
UseLuciferaseExpressionMammalianPromoterMyogeninAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Vimentin-7
Plasmid#56439PurposeLocalization: Intermediate Filaments, Excitation: 488, Emission: 507DepositorAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Alu-Nicn1
Plasmid#92344PurposePlasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.DepositorInsertNicn1 gene derived Alu element containing 3'UTR (NICN1 Human)
ExpressionMammalianPromoterCMVAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1
Plasmid#218796PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1
ExpressionMammalianAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S33Y Beta-catenin
Plasmid#19286PurposeMammalian expression of human beta-catenin with Flag tag and S33Y mutationDepositorInsertbeta-catenin (CTNNB1 Human)
TagsFlagExpressionMammalianMutationS33Y (presumptive GSK3β phosphorylation site)Available SinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
TS module
Plasmid#26021DepositorInsertTension Sensor module
ExpressionMammalianAvailable SinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hERalpha
Plasmid#101141PurposeMammalian expression of full-length human estrogen receptor-alphaDepositorAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA Nprl3 pRK5
Plasmid#46330DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
negMaLionG
Plasmid#113907Purposenegative control for Green ATP sensorDepositorInsertnegMaLionG
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
K44A HA-dynamin 1 pcDNA3.1
Plasmid#34683DepositorAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable SinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
Plasmid#207861PurposeAAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
UseAAVMutationSee ManuscriptPromoterCbhAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)
Plasmid#175573PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYLL201
Plasmid#53454PurposeExpresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)InsertHA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only
