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CMV-Hylight

Plasmid

#193447

Purpose

A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

Depositor

Richard Goodman

Publication

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Hylight Biosensor

Expression

Mammalian

Promoter

CMV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP

Plasmid

#32141

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

KDRT> STOP STOP KDRT>

Expression

Insect

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Publication

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FG12

Plasmid

#14884

Depositor

David Baltimore

Publication

Qin et al Proc Natl Acad Sci U S A. 2003 Jan 7. 10

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA

Plasmid

#191217

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Alpha (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

Del 69-70 Del 144 N501Y A570D D614G P681H T716I S…

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA

Plasmid

#191219

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Delta (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

T19R 156del 157del R158G L452R T478K D614G P681R …

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA

Plasmid

#191220

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Lambda (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

G75V T76I Del 246-252 L452Q F490S D614G T859N

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2P-WPRE

Plasmid

#179460

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has service

AAV1

Insert

JEDI-2P

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pClusterin-ERT2CreERT2

Plasmid

#14796

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Publication

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Publication

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pαH-S-GSAS-B.1.351.v2

Plasmid

#173786

Purpose

Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2

Depositor

Priyamvada Acharya

Publication

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Publication

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBAD33-mf-lon

Plasmid

#21867

Depositor

Robert Sauer

Publication

Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

Lon (Mfl404 Mesoplasma florum)

Expression

Bacterial

Mutation

all TGA codons changed to TGG codons

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLDLR-Luc mutSRE

Plasmid

#14945

Depositor

Axel Nohturfft

Publication

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

LDLR promoter mut SRE (LDLR Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Point mutation in the SRE-1 sequence of the LDL r…

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Stat4 pRc/CMV

Plasmid

#8711

Depositor

Jim Darnell

Publication

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat4 (Stat4 Mouse)

Expression

Mammalian

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLenti-Sa-gRNA-puro

Plasmid

#191652

Purpose

Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

Depositor

Moshe Szyf

Publication

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

S. aureus gRNAscr

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag DLC1

Plasmid

#24265

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Tags

Flag

Expression

Mammalian

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP RABV-G

Plasmid

#31833

Depositor

Connie Cepko

Publication

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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