We narrowed to 18,431 results for: Met

1. pFAB808

   Plasmid

   #47853

   Purpose

   BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rrnB T1 terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO8

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-26

   Plasmid

   #172751

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-26
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ-Myr-PTEN

   Plasmid

   #206983

   Purpose

   Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   N-terminal myristoylation site

   Expression

   Mammalian

   Promoter

   tet-on promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1D1R1

   Plasmid

   #97323

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1R1
   

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of R residue after T25

   Promoter

   T7

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK1169: CAG-DmrC-NLS-3xFLAG-P65

   Plasmid

   #104564

   Purpose

   Mammalian expression vector for DmrC fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   DmrC-p65
   

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Clover-pBAD

   Plasmid

   #54575

   Purpose

   Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   6xHis and Clover

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pET21-pelB-LaG-11

   Plasmid

   #172762

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-11
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP-PCAF-C2

    Plasmid

    #134572

    Purpose

    Expresses YFP-PCAF fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    PCAF (Kat2b Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV/3' Box_(GLuc)_INT

    Plasmid

    #68436

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC118-mCtnnb1-FLIP-Puro

    Plasmid

    #84540

    Purpose

    CRISPR - FLIP targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

    Plasmid

    #136916

    Purpose

    Expresses ChR2(H134R)-EYFP in Cre-negative cells

    Depositor

    Ofer Yizhar

    Article

    Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Mutation

    H134R in ChR2

    Promoter

    Human Synapsin1

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. peCALWY-5

    Plasmid

    #22237

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-5 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    C416S mutation identical to peCALWY-4, but linke…

    Available Since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

18. XRCC1-c003

    Plasmid

    #173090

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    XRCC1 (XRCC1 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E315-P406

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. peCALWY-3

    Plasmid

    #22235

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-3 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    Similar to eCALWY-1, but with a linkerlength of 3…

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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