Addgene

We narrowed to 26,416 results for: CAL

Showing: 7081 - 7120 of 26416 results

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SH3BGR_pCSdest

Plasmid

#53932

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SH3BGR (SH3BGR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Use

Tags

Knob

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

Plasmid

#70210

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pNB774

Plasmid

#60114

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-1-830

Plasmid

#40963

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 1-830

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE-100u

Plasmid

#13539

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Use

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pHH0103_TLN1_IRS

Plasmid

#109938

Purpose

Protein expression and purification of TLN1_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TLN1_IRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pANY3

Plasmid

#112197

Purpose

This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pYFP-x-1

Plasmid

#140142

Purpose

Evaluating information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Use

Tags

Expression

Bacterial

Mutation

D37, I38, and N39 are changed to nonsense (stop) …

Promoter

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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RWDD2B_pCSdest

Plasmid

#53866

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RWDD2B (RWDD2B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MTK3_004

Plasmid

#123720

Purpose

Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Hygro Resistance

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_005

Plasmid

#123721

Purpose

Encodes ble (Zeocin resistance) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Zeocin Resistance

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A

Plasmid

#19281

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc (Daxx Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
MTK2_026

Plasmid

#123718

Purpose

Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSFFVc

Use

Tags

Expression

Mammalian

Mutation

Crippled Kozac sequence

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK581] pRK5 HA-SLC38A9N

Plasmid

#136141

Purpose

mammalian expression of SLC38A9 N term fragment (1-119)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-R1868*-3xFLAG

Plasmid

#130679

Purpose

Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 R1868* (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R1868_V2005delinsX

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pPAP001

Plasmid

#153488

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_OctaHis-GFP11

Plasmid

#153509

Purpose

OctaHis-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

OctaHis-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_TRIP6_LIM

Plasmid

#109941

Purpose

Protein expression and purification of TRIP6_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TRIP6_LIM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

Plasmid

#87586

Purpose

PV7 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF-yibDp-matB

Plasmid

#134597

Purpose

Low Phosphate inducible matB (malonyl-CoA synthetase)

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

Malonyl CoA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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3xDHFR-myc

Plasmid

#20216

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
SP_gRNA_pUC19_N_FancF_Left

Plasmid

#79892

Purpose

Human gRNA expression vector targeting FANCF (site 1 left)

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

FancF_Site_1_Left

Use

CRISPR

Tags

None

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAM4645

Plasmid

#85124

Purpose

NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiBC-KaiBC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Flag-mPar6a.PIG

Plasmid

#24599

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pET_GFP-SZ2

Plasmid

#124931

Purpose

Expression of GFP-SZ2

Depositor

Daniel Hammer, Matthew Good, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

GFP-SYNZIP2

Use

Tags

GFP and His

Expression

Bacterial

Mutation

SZ2 domain

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RAN_Ras

Plasmid

#109936

Purpose

Protein expression and purification of RAN_Ras

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAN_Ras

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSγ

Plasmid

#68284

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
MTK1_018

Plasmid

#123681

Purpose

Encodes ConL8 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
SCAF4_pCSdest

Plasmid

#53797

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SCAF4 (SCAF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pX330-sgRNA_Drosha_1

Plasmid

#79860

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_2

Plasmid

#79861

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
Xlim-1 (Lhx1) XH32-3

Plasmid

#74135

Purpose

Basic cDNA clone for the Xlim-1/Lhx1 gene. In Bluescript BS KS+. To prepare RNA for Sense: BamHI/T3; for antisense: XhoI/T7

Depositor

Igor Dawid

Article

Taira et al Genes Dev. 1992 Mar;6(3):356-66.

Insert

lhx1 (lhx1 Frog)

Use

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
MTK3b_029

Plasmid

#123807

Purpose

Encodes Tet-On 3G transactivator protein as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TET3G

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
POT5-CYC1p-ycrtYB-ADH1t

Plasmid

#65360

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtYB-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSε

Plasmid

#68286

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Dnm2a

Plasmid

#109571

Purpose

Multisite gateway vector for 3' tagging with Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2a

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7081 - 7120 of 26416 results

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