We narrowed to 29,546 results for: REP

1. pGK:HygroR-CMV:mKO2-C_UTR

   Plasmid

   #68481

   Purpose

   Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKO2-C_UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30a-SRAP

   Plasmid

   #84524

   Purpose

   Expresses murine SRAP domain (250 aa) in bacteria

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   SRAP domain (Hmces Mouse)
   

   Use

   Tags

   6xHis and S-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCytERM-EBFP2

   Plasmid

   #184939

   Purpose

   ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   CytERM-EBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT-mWasabi-G

   Plasmid

   #164170

   Purpose

   Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mWasabi
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_VNp-DARPinOFF7

   Plasmid

   #182397

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-DARPinOFF7
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD765 HMGCR 3'UTR

   Plasmid

   #105766

   Purpose

   Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

   Depositor

   Joseph Ziegelbauer

   Article

   Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

   Insert

   HMGCR 3'UTR (HMGCR Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-eDIO-ChR2-EYFP

   Plasmid

   #104387

   Purpose

   PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Channelrhodopsin 2
   

   Use

   Cre/Lox; Piggybac

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E134R

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP_E1770Q-talin1 (1-2541)

   Plasmid

   #166121

   Purpose

   For expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   E1770Q-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E1770Q

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xhyb PylT sfGFP 150TAG

   Plasmid

   #154771

   Purpose

   plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T-1-3xMyc-ERK2(K52R)

    Plasmid

    #129228

    Purpose

    Encodes Myc tagged human ERK2(K52R) for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    ERK2(K52R) (Mapk1 Rat)
    

    Use

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    Changed Lysine 52 to Arginine

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ptCMV-153/47-VR-NG

    Plasmid

    #50704

    Purpose

    Final destination vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    TALEN

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC-mWasabi-G4

    Plasmid

    #164168

    Purpose

    Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ40 GADD34 (214-513aa)

    Plasmid

    #78095

    Purpose

    mammalian expression of GADD34 214-513 aa

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    expresses aa 214-513

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pPuroR-MCS-GAL4-MiniWhite

    Plasmid

    #165890

    Purpose

    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p.UTA.2.0-GL-2

    Plasmid

    #82440

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mutation

    The complementary region for GL2 siRNA control wa…

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p63RhoGEF580-mVenus-N1

    Plasmid

    #84338

    Purpose

    Expresses p63 (580 a.a.) tagged with mVenus

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

    Insert

    p63RhoGEF (580 aa) (ARHGEF25 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. myc-hIFITM3-F63Q

    Plasmid

    #104363

    Purpose

    Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutated the following amino acid: F63Q

    Promoter

    CMV

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PWPXLD-GFP-Numb-S276D

    Plasmid

    #133752

    Purpose

    Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    NUMB endocytic adaptor protein (Numb Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    serine 276 converted to aspartic acid

    Promoter

    EF1-alpha

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. epiCRISPRe

    Plasmid

    #135962

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pN1-mCherry-NKD2

    Plasmid

    #176641

    Purpose

    Expresses NKD2-mCherry fusion protein in mammalian cells

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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