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We narrowed to 2,760 results for: FAS

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Showing: 851 - 900 of 2760 results

  2. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124807-plasmid-map-sequence-id-251832

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  3. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid
    #202555
    Purpose
    Synaptotagmin-1 sgRNA2 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202555-plasmid-map-sequence-id-404691

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  4. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202556-plasmid-map-sequence-id-404689

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  5. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202554-plasmid-map-sequence-id-404687

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  6. pHybr_r2a>Fab-srt-his

    Plasmid
    #124810
    Purpose
    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    rat IgG2a Hinge-G4S-Sortag-Histag
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124810-plasmid-map-sequence-id-243698

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  7. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  8. pGL3-basic-hFX-promotor(2kb, distal)

    Plasmid
    #14981
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Mutation
    includes distal fragment of human frataxin promot…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  12. pGL3-basic-hFX-promotor(2,5kb)

    Plasmid
    #14980
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Mutation
    start codon for human frataxin gene has been muta…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  13. pGL3-basic-hFX-promotor(1,3kb)

    Plasmid
    #14979
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Mutation
    start codon for human frataxin gene has been muta…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  14. pGL3-basic-hFX-promotor(1kb)

    Plasmid
    #14978
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Mutation
    start codon for human frataxin gene has been muta…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  15. pBlue-hFX(I154F)(AA88)-HA

    Plasmid
    #15403
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1, HA, and HIS
    Expression
    Bacterial
    Mutation
    contains cDNA coding sequence for amino acids 88-…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  16. pET28(a)-hFX(I154F)-HA

    Plasmid
    #14992
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1, HA, and HIS
    Expression
    Bacterial
    Mutation
    contains cDNA coding sequence for amino acids 88-…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  17. pSIREN-RetroQ-BPTF-sh28

    Plasmid
    #73669
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA 28 to BPTF (BPTF, Bptf Mouse, Human)
    Use
    Retroviral
    Available Since
    March 22, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73669-plasmid-map-sequence-id-138080

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  18. pSIREN-RetroQ-BPTF-sh27

    Plasmid
    #73668
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA #27 to BPTF (BPTF, Bptf Mouse, Human)
    Use
    Retroviral
    Available Since
    April 13, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73668-plasmid-map-sequence-id-141990

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  20. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  28. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid
    #122850
    Purpose
    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122850-plasmid-map-sequence-id-238544

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  38. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid
    #122849
    Purpose
    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT) (Fasn Mouse)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122849-plasmid-map-sequence-id-238542

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Showing: 851 - 900 of 2760 results