We narrowed to 1,879 results for: Ipp

1. pDEST17-LAP3

   Plasmid

   #217661

   Purpose

   For protein purification from Ecoli

   Depositor

   Karla Feijs

   Article

   Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

   Insert

   LAP3 (LAP3 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR221-LAP3

   Plasmid

   #217660

   Purpose

   For gateway cloning of LAP3

   Depositor

   Karla Feijs

   Article

   Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

   Insert

   LAP3 (LAP3 Human)
   

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACYCDuet-1:ChrA-C63S

   Plasmid

   #225080

   Purpose

   Expresses precursor peptide ChrA-C63S in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA-C63S
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C63S

   Promoter

   T7

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDFDuet-1:ChrH-ChrI

   Plasmid

   #225083

   Purpose

   Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrH
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-APOBEC1-YTH-EGFP

   Plasmid

   #209322

   Purpose

   AAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-YTH-HA
   

   Use

   AAV

   Tags

   HA

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EGFP-APOBEC1-YTH

   Plasmid

   #209319

   Purpose

   AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-YTH-HA
   

   Use

   AAV

   Tags

   HA

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

   Plasmid

   #179717

   Purpose

   Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMx/ATP10D(E215Q)-HA

   Plasmid

   #209260

   Purpose

   Mammalian expression of ATP10D mutant E215Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10D (ATP10D Human)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   E215Q

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMx/ATP8B4-HA

   Plasmid

   #209229

   Purpose

   Mammalian expression of ATP8B4

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8B4 (ATP8B4 Human)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG/ATP10B(E210Q)-HA

    Plasmid

    #209247

    Purpose

    Mammalian expression of ATP10B mutant E210Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10B (ATP10B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E210Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMx/ATP10B-HA

    Plasmid

    #209233

    Purpose

    Mammalian expression of ATP10B

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMx/ATP10B(E210Q)-HA

    Plasmid

    #209259

    Purpose

    Mammalian expression of ATP10B mutant E210Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    E210Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMx/ATP10A-HA

    Plasmid

    #209232

    Purpose

    Mammalian expression of ATP10A

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10A (ATP10A Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMx/ATP8B3-HA

    Plasmid

    #209228

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPV-C1-crRNA-cloning-EF1a-BA

    Plasmid

    #204623

    Purpose

    piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

    Plasmid

    #158771

    Purpose

    Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO/MACROD1

    Plasmid

    #172578

    Purpose

    For generation of FlpIn lines with untagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

    Plasmid

    #172583

    Purpose

    For transient expression of C-terminal BirA-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

    Plasmid

    #140223

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
    

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-myc-Sema4A RNAiR

    Plasmid

    #115172

    Purpose

    expresses RNAi-resistant Sema4A with N-terminal myc tag

    Depositor

    Suzanne Paradis

    Article

    McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

    Insert

    Sema4A (Sema4a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Silent mutation between amino acids in ORF (401-4…

    Promoter

    CMV

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCML 313

    Plasmid

    #98586

    Purpose

    pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    csrB
    

    Expression

    Bacterial

    Promoter

    pLacO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCML 314

    Plasmid

    #98584

    Purpose

    pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Inserts

    CsrA
    csrB
    

    Expression

    Bacterial

    Promoter

    pLacO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tdEos-Cx43-7

    Plasmid

    #57603

    Purpose

    Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Cx43
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pRSETC PITPbeta N89F

    Plasmid

    #58279

    Purpose

    bacterial expression of PITP beta N89F mutant

    Depositor

    Shamshad Cockcroft

    Article

    Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

    Insert

    PITP beta (PITPNB Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N89F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pFA6a-His3MX6-PGAL1-GST

    Plasmid

    #41613

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid

    #158768

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Mutation

    Flexible linkers between the protease and msGFP a…

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF(UG) hSyn SYP-deltaTMD SYT7alpha

    Plasmid

    #179718

    Purpose

    Lentiviral expression of synaptic vesicle-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

    Plasmid

    #179719

    Purpose

    Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179721

    Purpose

    Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

    Plasmid

    #179722

    Purpose

    Lentiviral expression of lysosome-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179723

    Purpose

    Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pF(UG) U6-SYT7 sgRNA 777 HaloTag

    Plasmid

    #179724

    Purpose

    U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    sgRNA and HaloTag
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pF(UG) CamKII sf iGluSnFR S72A

    Plasmid

    #179726

    Purpose

    Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    iGluSnFR
    

    Use

    Lentiviral

    Mutation

    S72A

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Promoter

    CBh; U6

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124428

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pINDUCER21-puro_TAOK1

    Plasmid

    #209777

    Purpose

    doxycycline-inducible expression of TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

    Plasmid

    #170229

    Purpose

    Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Q64L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEF1a-mMLH1dn

    Plasmid

    #174825

    Purpose

    Mammalian expression of mouse MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    mouse MLH1dn (codon optimized)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 758-760

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCCL/IFNB1-d2eGFP-3’UTR

    Plasmid

    #180232

    Purpose

    Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

    Insert

    d2eGFP
    

    Use

    Lentiviral

    Promoter

    1 kb upstream of IFNB1 CDS

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200402

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pmax pona 12xTRICK 24xMS2SL

    Plasmid

    #64542

    Purpose

    TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Tags

    24xMS2 stem loops, HA, NLS, and dNP799

    Expression

    Mammalian

    Promoter

    ponA

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid

    #200410

    Purpose

    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor detecting iRFP720
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pmScarlet-HSP70

    Plasmid

    #163790

    Purpose

    Expresses mScarlet-Hsp70 in mammalian cells

    Depositor

    Vincent Timmerman

    Article

    Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

    Insert

    HSP70 (HSPA1A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pFA6a-His3MX6-PGAL1-GFP

    Plasmid

    #41616

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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