We narrowed to 18,247 results for: multi
-
Plasmid#55641PurposeFlp-Dependent EYFPDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, and AAV RetrogradeInsertEYFP
UseAAVExpressionMammalianPromoterEf1aAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-Cetn1/1
Plasmid#50718PurposepX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCetn1 sgRNA1
humanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBh and hU6Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-dV-IgG1/κ
Plasmid#52213PurposeHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region
ExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralExpressionInsectPromoterCMV and U6 and H1Available sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2 HRS-RFP
Plasmid#29685DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
TagsRFPExpressionMammalianAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-myc3-bTrCP
Plasmid#20718DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbeta-transducin repeat containing (BTRC) (Btrc Mouse)
TagsMyc3ExpressionMammalianMutation*Available sinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-FLEX-rev-ChR2(H134R)-mCherry
Plasmid#18916PurposeCre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitationDepositorInsertChannelrhodopsin 2-mCherry
UseAAV and Cre/Lox; Adeno-associated virusTagsmCherryMutationH134RPromoterCAGAvailable sinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP- SEC61B
Plasmid#121159PurposeMammalian expression of GFP tagged SEC61B, used as a general ER markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEC61B (SEC61B Human)
ExpressionMammalianAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBA439
Plasmid#85967PurposePerturb-seq vector backboneDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1/hChR2(H134R)-EYFP
Plasmid#20940PurposeMammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertchannelrhodopsin-2
ExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
Plasmid#65417PurposeAAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.DepositorHas serviceAAV8 and AAV RetrogradeInserthSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
UseAAVTagsHA and mCitrinePromoterhSynAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
TagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDESTtol2pACrymCherry
Plasmid#64023PurposeGateway destination vector with zebrafish aA-crystallin promoter driving mCherry in lensDepositorInsertmCherry
UseGateway CloningPromoteralpha-Acrystallin promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGateway CloningPromoteralpha-Acrystallin promoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
ExpressionBacterialMutationE32KAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by