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Anti-CALB2/calretinin [L122/68R-1]

Plasmid

#206690

Purpose

Mammalian Expression Plasmid of anti-CALB2/calretinin (Human). Derived from hybridoma L122/68-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CALB2/calretinin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-CALB2/calretinin [L122/68R-2b]

Plasmid

#206641

Purpose

Mammalian Expression Plasmid of anti-CALB2/calretinin (Human). Derived from hybridoma L122/68-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CALB2/calretinin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-β-catenin_S23A,T40A,T41A,T112A

Plasmid

#190824

Purpose

For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

β-catenin (CTNNB1 Chicken)

Use

Tags

GFP

Expression

Mammalian

Mutation

S23A, T40A, T41A, T112A

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

Plasmid

#168787

Purpose

Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pZDonor-U6-myogenin stop codon gRNA

Plasmid

#69549

Purpose

Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Insert

Myogenin C terminus-targeting guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-human beta-catenin full length

Plasmid

#132609

Purpose

expresses human beta-catenin full length in E. coli

Depositor

Wenqing Xu

Article

Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

Insert

beta-catenin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (1B)

Plasmid

#29653

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (u-msfGFP)

Plasmid

#29772

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

SiriusGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (u-tdTomato)

Plasmid

#86926

Purpose

pET LIC cloning vector for yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

tdTomato

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET mCherry LIC cloning vector (u-mCherry)

Plasmid

#29769

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (2B-T)

Plasmid

#29666

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV LIC cloning vector (4B)

Plasmid

#30115

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-TEV

Expression

Insect

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (1R)

Plasmid

#29664

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Strep II and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 LIC cloning vector (2Bc-T)

Plasmid

#37236

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Use

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only