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Plasmid#53788DepositorInsertTPTE (TPTE Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBW206J106-gfp
Plasmid#78645PurposepSB3K3 carrying J106-30gfp-tDepositorInsertJ106-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW201J109-gfp
Plasmid#78640PurposepSB3K3 carrying J109-30gfp-tDepositorInsertJ109-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW204J115-gfp
Plasmid#78643PurposepSB3K3 carrying J115-30gfp-tDepositorInsertJ115-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-CYC1p-ycrtE-ADH1t
Plasmid#65354PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertCYC1p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT4-CYC1p-ycrtI-ADH1t
Plasmid#65357PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertCYC1p-ycrtI-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LexAop2->Bxb1.STOP>myr::4xSNAPf
Plasmid#87640PurposeFor creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC20Cerulean3-Cdc8
Plasmid#99357PurposeBacterial expression of Cerulean3 fused to the 5' of cDNA of the fission yeast tropomyosin, cdc8.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsCerulean3 and His6-Gly3ExpressionBacterialMutationPromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Hoxc6 (CT#541)
Plasmid#13986DepositorInsertHoxc6 (HOXC6 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNB775
Plasmid#60115PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertYeLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…ExpressionMutationPromoterAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB776
Plasmid#60117PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertRdLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…ExpressionMutationPromoterAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-KS-Nrg3-ISH
Plasmid#87669PurposeIn situ probe for mouse Nrg3DepositorInsertNrg3 (Nrg3 Mouse)
UseTagsExpressionBacterialMutationPromoterT7/M13Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
SI5-IGSF21a
Plasmid#36115DepositorInsertIGSF21a mouse monoclonal antibody (igsf21a Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX4-AS-short (pcDNA3.1+)
Plasmid#75210PurposeMammalian expression of SLC24A4DepositorInsertSLC24A4 (SLC24A4 Human)
UseTagsExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mRFP-Syx2
Plasmid#35704DepositorInsertSyx2 (Plekhg5 Mouse)
UseTagsmRFPExpressionMammalianMutationR205KPromoterCMVAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
XE75 Beta-catenin Delta N-9 GFP
Plasmid#16831DepositorInsertbeta-catenin Delta N - 9 GFP (ctnnb1.L Frog)
UseXenopus expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)
Plasmid#70213Purpose1 Part of 4-part switchDepositorInsertlacI
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
POT4-TEF2p-ycrtI-ADH1t
Plasmid#65356PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtI-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
140_pSKB3-GBD-LUMP
Plasmid#65972PurposeBacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42DepositorInsertLUMP
UseTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSν
Plasmid#68294PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 5xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSβ
Plasmid#68283PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 1xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
SI12-Lrrc3b
Plasmid#46302DepositorInsertLrrc3b mouse monoclonal antibody (lrrc3b Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-cfRabin8_4
Plasmid#26713DepositorInsertdog Rabin8 RNAi
UseLentiviral and RNAiTagsExpressionMammalianMutationRNAi to dog Rabin8 alpha isoform.PromoterAvailable sinceDec. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
XE73 TM Beta-catenin 1-myc in CS2+
Plasmid#16834DepositorInsertTM beta-catenin 1-myc (ctnnb1.L Frog)
UseXenopus expressionTagsc-mycExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
LSM102 CMV-TO-CX3CR1-10aa-PCP (FLP-IN)
Plasmid#233404PurposeCX3CR1-V2-PCP with a 10 amino acid linker and V2 tailDepositorInsertCX3CR1-PCP
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceJune 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
P-Bf-fusA2
Plasmid#235658PurposeReports Cur activity in Bacteroides fragilisDepositorInsertBacteroides fragilis fusA2 promoter
UseTagsExpressionMutationThe B. thetaiotaomicron fusA2 promoter lacking &q…PromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Mito-PAmKate
Plasmid#238078PurposeExpresses photoactivable fluorescent reporter for visualizing mitochondrial networkDepositorInsertPAmKate
UseTagsMitoExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_mutCKAR3
Plasmid#238414PurposeAAV construct with CKAR3 mutant expression under a human synapsin promotor and WPRE3 translation enhancer.DepositorInsertphosphorylation deficient mutant of CKAR3 (T to A)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CKAR3
Plasmid#238413PurposeAAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.DepositorInsertCKAR3 (PKC sensor)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
RNF168 C-terminal sgRNA
Plasmid#207100PurposepX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.DepositorInsertGAGATGCACAAAGTAAGGCC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
RIF C-terminal sgRNA
Plasmid#207096PurposepX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.DepositorInsertAATACTAAATAGAATTTTCA
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
REV7 C-terminal sgRNA
Plasmid#207095PurposepX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.DepositorInsertGCTCATAAAGGCAGCTGAGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT08
Plasmid#223380PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT09
Plasmid#223381PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT10
Plasmid#223382PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT01
Plasmid#223373PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT02
Plasmid#223374PurposeT-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only