We narrowed to 13,571 results for: GAN

1. pSTAP-seq_human-4xUAS

   Plasmid

   #125150

   Purpose

   Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   4 x upstream activating sequence (UAS)
   

   Use

   Stap-seq screening vector

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

   Plasmid

   #196195

   Purpose

   Editing human TSC2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-sapphireAKAR-NLS

   Plasmid

   #118467

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR-NLS
   

   Tags

   Nuclear localization signal (NLS)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM103

   Plasmid

   #133664

   Purpose

   Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR02

   Plasmid

   #69149

   Purpose

   Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   eGFP
   

   Use

   Cre/Lox; Mossci

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   rps-27

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv2-dADAM17 (#1)

   Plasmid

   #177259

   Purpose

   A knockout vector for the dog Adam17

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM100

   Plasmid

   #133662

   Purpose

   Integration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

    Plasmid

    #164040

    Purpose

    Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    sgTS5-sgTS6-sgTS7
    

    Use

    CRISPR

    Promoter

    mouse U6

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZJOM96

    Plasmid

    #133663

    Purpose

    Integration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC26-mTagBFP2 (SEC26 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

    Plasmid

    #37602

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I C820A (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C820A

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pKmyc-Borg3

    Plasmid

    #141074

    Purpose

    Expresses Borg3 (Cdc42ep5) in mammalian cells

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

    Insert

    Borg3 (Cdc42ep5 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOD_A0402

    Plasmid

    #91009

    Purpose

    Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Mutation

    D10A, H840A

    Promoter

    AtUbi10

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

    Plasmid

    #216380

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lyn-CFP-GAI(1-92)

    Plasmid

    #37311

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

    Plasmid

    #32483

    Purpose

    Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    ChR2HA-2a-PSAML141F,Y115F:GlyR
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    ChR2 2A

    Promoter

    CAG

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIREN-RetroQ-shLuc

    Plasmid

    #73666

    Purpose

    pSRIEN-RetroQ vector containing control sequence luciferase

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Available Since

    April 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

    Plasmid

    #112921

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Bgh-GFP-Farpl

    Plasmid

    #115501

    Purpose

    expression of rat Farp1

    Depositor

    Thomas Biederer

    Article

    Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

    Insert

    Farp1 (Farp1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    chicken beta-actin

    Available Since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

22. Hoxb7 promoter

    Plasmid

    #21294

    Depositor

    Frank Costantini

    Article

    Srinivas et al Development. 1999 Apr . 126(7):1375-86.

    Insert

    Hoxb7 promoter (Hoxb7 Mouse)
    

    Use

    Promoter can be used to make transgenic mice

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TF-bio-His

    Plasmid

    #52184

    Purpose

    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TF (TF Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MTK0_057

    Plasmid

    #123950

    Purpose

    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI LP on hAAVS1 (Hygro::mRuby)
    

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKD280.7

    Plasmid

    #173172

    Purpose

    Expresses SO_4387

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    SO_4387
    

    Expression

    Bacterial

    Promoter

    J23107

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBa.FRB-3myc-KAP3B

    Plasmid

    #193939

    Purpose

    Mammalian expression of KAP 3B

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    KAP 3B (KIFAP3 Human)
    

    Tags

    FRB

    Expression

    Mammalian

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+)-ExRai-AKAR(T/A)

    Plasmid

    #118406

    Purpose

    Negative-control mutant for ExRai-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. K401-iLid

    Plasmid

    #122484

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    Kinesin 1-401 iLid His tag
    

    Tags

    His6 and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Promoter

    T7

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-mKate-P2A-APEX2-NS

    Plasmid

    #67670

    Purpose

    Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-NS
    

    Use

    Gateway multisite middle entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR221_SLC22A3

    Plasmid

    #132024

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A3 (SLC22A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

    Plasmid

    #139788

    Purpose

    Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MTK0_047

    Plasmid

    #123977

    Purpose

    Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hCLYBL CAS9 Destination - HygroR
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PhyB-mCherry-NLS

    Plasmid

    #51571

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    NLS and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSF2C-A-RFP

    Plasmid

    #172432

    Purpose

    StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]

    Plasmid

    #66781

    Purpose

    Gly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG

    Depositor

    Yishi Jin

    Article

    Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    miniSOG
    

    Tags

    tomm-20 (N terminal 55 aa)

    Expression

    Worm

    Mutation

    G426C

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-17b_Unc104-KIF5B-HALO

    Plasmid

    #169996

    Purpose

    Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

    Depositor

    Ron Vale

    Article

    Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

    Insert

    Kinesin-like protein Unc-104 fused with KIF5B
    

    Tags

    HALO-tag, 6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHH0103_S100A11_S-100

    Plasmid

    #109804

    Purpose

    Protein expression and purification of S100A11_S-100

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    S100A11_S-100 (S100A11 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge