We narrowed to 17,931 results for: COMP

1. p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

   Plasmid

   #37606

   Depositor

   Peter Howley

   Article

   Martinez-Noel et al Mol Cell Biol. 2012 May 29.

   Insert

   E6AP III C840A (UBE3A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C840A

   Promoter

   CMV tet regulatable

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

2. vsv-CenpB 1-166-Sgo1 N61I-GFP

   Plasmid

   #108499

   Purpose

   expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

   Depositor

   Susanne Lens

   Article

   Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

   Insert

   CenpB-Shugoshin 1 (SGO1 Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CenpB 1-166 fusion before Sgo1, no PP2A binding N…

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 HRPT2 L64P

   Plasmid

   #11052

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   HRPT2 L64P (CDC73 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L64P

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

   Plasmid

   #172581

   Purpose

   For transient expression of C-terminal BirA-tagged MACROD1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSUPERpuro-BglII-Hs4E-T_AC

   Plasmid

   #148445

   Purpose

   Mammalian Expression of Hs4E-T-shRNA

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   Hs4E-T-shRNA (EIF4ENIF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. phumanCas9 (GB0575)

   Plasmid

   #68206

   Purpose

   Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO HIS DNAJA4

   Plasmid

   #19548

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_PSTPIP1-1/1

   Plasmid

   #91453

   Purpose

   Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PSTPIP1-1/1 (PSTPIP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFL His-SDS22

   Plasmid

   #113512

   Purpose

   Expression of His-SDS22 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   SDS22 (PPP1R7 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R4pGWB401+Citrine

    Plasmid

    #128432

    Purpose

    Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Citrine, yellow fluorescence protein

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUASp-FMW-attB-AthHen1

    Plasmid

    #104957

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    pTrasposase promoter

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-ABKAR-Kras

    Plasmid

    #84637

    Purpose

    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, C…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJZC39

    Plasmid

    #62333

    Purpose

    sgRNA + 1x PP7 with mCherry for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x PP7
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ETV7_A86D_pet28a

    Plasmid

    #131657

    Purpose

    Bacterial expression of ETS gene with A86D mutation for solubility

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV7 A86D (ETV7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Alanine 86 to Aspartic Acid

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTNos:BASTA:PNos (GB0023)

    Plasmid

    #68211

    Purpose

    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    bar gene
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    PNos

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3xFLAG-THAP11-rescue

    Plasmid

    #37001

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 (THAP11 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Mutations were generated at codons 273-275 conver…

    Promoter

    Available since

    May 21, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pHH0103_PIK3R1-1/1

    Plasmid

    #91274

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R1-1/1 (PIK3R1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEBTetBl-CLIP-plk4

    Plasmid

    #136837

    Purpose

    Mammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-plk4 (PLK4 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid

    #82469

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mRNA capping enzyme (Rngtt Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    changed Lysine 294 to Alanine

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt Mouse, HIV)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV10/3xflag-Clock L57E

    Plasmid

    #47337

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L57E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pCMV10/3xflag-Clock L74E

    Plasmid

    #47338

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L74E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid

    #84631

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ERKsub(TA)DD-Venus-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Mutated target Thr residue to Ala.

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_NEDD9-1/1

    Plasmid

    #91498

    Purpose

    Protein expression and purification of human SH3 domain construct NEDD9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEDD9-1/1 (NEDD9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. px330 CTCF gRNA

    Plasmid

    #165588

    Purpose

    Insertion of CTCF degon

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    CTCF (CTCF Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ETV3_pet28a

    Plasmid

    #131646

    Purpose

    Bacterial expression of ETS gene ETV3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV3 (ETV3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. px330 CBP gRNA

    Plasmid

    #165587

    Purpose

    insertin of CBP degrom

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAVsc.U6-sgGTTG.CMV/CB-Gluc

    Plasmid

    #121512

    Purpose

    Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgGTTG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKD265

    Plasmid

    #125095

    Purpose

    Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    torR(REC)-psdR(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSR322

    Plasmid

    #125116

    Purpose

    Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    uhpA(REC)-ydfI(DBD)131
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSR40.15

    Plasmid

    #125082

    Purpose

    Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-ccaR(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSR40.29

    Plasmid

    #125088

    Purpose

    Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-ccaR(DBD)139
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABEhygro-Flag-ZO-2-1st-PDZ-mut

    Plasmid

    #27474

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K38E, F44H, I46E and V48E

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pDECKO-mCherry TFRC_B

    Plasmid

    #78544

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA5 HA-CCDC95

    Plasmid

    #19795

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    CCDC95 (INO80E Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pCMV10/3xflag-Clock V315R

    Plasmid

    #47342

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    V315R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

38. psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

    Plasmid

    #61792

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhag 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

39. 5' Del 3

    Plasmid

    #16051

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -927 to +88 of promoter

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pLacIBD::SDRX (GB0673)

    Plasmid

    #68183

    Purpose

    Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    LacI Binding Domain fused to SRDX Transcriptional Repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHH0103_NOSTRIN-1/1-SV1/2

    Plasmid

    #91323

    Purpose

    Protein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NOSTRIN-1/1-SV1/2 (NOSTRIN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV10/3xflag-Clock W284A

    Plasmid

    #47341

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pHH0103_SH3KBP1-1/3

    Plasmid

    #91555

    Purpose

    Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3KBP1-1/3 (SH3KBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-myc NES- mCE(K294A)

    Plasmid

    #82464

    Purpose

    Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt Mouse, HIV)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K294A

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. p2Flag CMV2-ZO-2-delta-N

    Plasmid

    #27419

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Deletion of coding nucleotides from 1-351. This r…

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

46. HOXA4 (murine) pET

    Plasmid

    #8488

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXA4 (Hoxa4 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Thr-2 changed to Ala-2 to form better ribosomal b…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

47. pHH0103_SH3RF2-3/3

    Plasmid

    #91281

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF2-3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF2-3/3 (SH3RF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMX-Flag-WIP 460 deletion

    Plasmid

    #11773

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP aa1-460 (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa461-503 deleted

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

49. MSCV-PM BRD7 sh1

    Plasmid

    #41927

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

50. pET19b-MED29

    Plasmid

    #15415

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED29 (MED29 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only