We narrowed to 9,202 results for: sacs

1. pICH-roGFP2-ORP1

   Plasmid

   #191677

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   redox(ro)GFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPCX roGFP2-Orp1

   Plasmid

   #64991

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. c-Flag pcDNA3

   Plasmid

   #20011

   Purpose

   This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCSUP35(1-253)

   Plasmid

   #1089

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acids 1 - 253 of SUP35

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-Lifeact-tdTomato

   Plasmid

   #64048

   Purpose

   Fluorescent reporter for F-actin labeling in living cells

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV-TRE-WT human MyoD-T2A-dsRedExpress2

   Plasmid

   #60628

   Purpose

   Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 mouse MyoD (MYOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPCX mito roGFP2-Orp1

   Plasmid

   #64992

   Purpose

   Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PMXS-NDI1

   Plasmid

   #72876

   Purpose

   expresses NDI1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

   Insert

   NDI1 (NDI1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211622

    Purpose

    Rhno1 S141A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S141A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-C1-ORP5deltaTMD-FKBP

    Plasmid

    #220073

    Purpose

    expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5deltaTMD (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deltaTMD

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-CMV-TFAM-BioID2-IRES-Puro

    Plasmid

    #211465

    Purpose

    Lentivirus backbone expressing human TFAM fused with BioID2

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    TFAM-BioID2
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS Omomyc

    Plasmid

    #113164

    Purpose

    Omomyc expression in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

    Insert

    Omomyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416GPD-Cerulean-VPS21

    Plasmid

    #18842

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    VPS21 (VPS21 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

16. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFSKm4-ATF1

    Plasmid

    #221133

    Purpose

    Formaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detection

    Depositor

    André Studart

    Article

    Dutto et al Adv Mater. 2024 Dec 10:e2412555. doi: 10

    Insert

    ATF1 (ATF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm4

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFW6000

    Plasmid

    #224215

    Purpose

    Bacteroides genomic editing tool; aTc inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tubP-FRT>GAL80-6-FRT>

    Plasmid

    #63173

    Purpose

    ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "Flp-Out" GAL80
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

    Plasmid

    #35152

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5, pTrc

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUltra-5HTP

    Plasmid

    #178042

    Purpose

    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T107C, P254T, C255A

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJAT17

    Plasmid

    #204298

    Purpose

    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PSD-95 FLAG

    Plasmid

    #15463

    Depositor

    Wei-dong Yao

    Article

    Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Plasmid

    #58442

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. R57C10-Flp2

    Plasmid

    #63172

    Purpose

    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only