We narrowed to 11,455 results for: ARIA

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pRc/CMV HA TrkB-T1

Plasmid

#39980

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB.T1 (Ntrk2 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

TrkB.T1 variant; endogenous signal sequence was r…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only
PKAcs-GGS4X-GFP

Plasmid

#108583

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRRG389-PC2

Plasmid

#99087

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone convertase 2 (PC2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

prohormone convertase 2 (PC2)

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG757-smedwi-1

Plasmid

#99074

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Smedwi-1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Smedwi-1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pML107-kanR468

Plasmid

#162041

Purpose

Expresses Cas9 and contains kanR468 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR468 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-Cre

Plasmid

#47117

Purpose

Can be used to in vitro transcribe Cre recombinase.

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

Cre

Promoter

Lac

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pHL 1903

Plasmid

#53020

Purpose

AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

GFP

Use

Synthetic Biology

Tags

AAV, Asp terminator, and fimS

Expression

Bacterial

Promoter

fimS

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pMS8: pTns(AvCAST)

Plasmid

#168141

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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CMV plasmid

Plasmid

#169739

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMS15: pTwinStrep-SUMO-AvTnsB

Plasmid

#168148

Purpose

AvTnsB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsB

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS16: pTwinStrep-SUMO-AvTnsC

Plasmid

#168149

Purpose

AvTnsC expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsC

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS14: pTwinStrep-SUMO-AvTnsA

Plasmid

#168147

Purpose

AvTnsA expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsA

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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MECP2 - R133C in pENTR

Plasmid

#216594

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

R133C

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pZH520

Plasmid

#102673

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-eAID-BlastR

Plasmid

#194062

Purpose

Mammalian expression of evoCjCas9 eAID

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pZH521

Plasmid

#102674

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pV1539

Plasmid

#111434

Purpose

Genedrive entry vector for Candida albicans

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS-myc

Plasmid

#11548

Depositor

Sandra Orsulic

Article

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

c-myc (MYC Human)

Use

Retroviral

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pZH517

Plasmid

#102670

Purpose

Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMP4516

Plasmid

#107775

Purpose

Expression of ECFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-ECFP in pBBR1-mcs5

Tags

pLacZ-ECFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR macroH2A1.2-C

Plasmid

#30512

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Gateway - donor vector

Mutation

NO STOP

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1+ HA-hAO7

Plasmid

#137840

Purpose

N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Tags

HA

Expression

Mammalian

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ Bmi1-cherry-HA

Plasmid

#30516

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

Bmi1 (Bmi1 Mouse)

Use

Xenopus/zebrafish expression

Tags

cherry and triple HA

Expression

Mammalian

Mutation

NO STOP

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCK760

Plasmid

#192643

Purpose

Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23110-sfGFP

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23110

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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CP1300-ABEmax

Plasmid

#119810

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZH519

Plasmid

#102672

Purpose

Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CMV+ plasmid with onboard mCherry cassette

Plasmid

#169741

Purpose

CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZH522

Plasmid

#102675

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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