We narrowed to 9,644 results for: SUB

1. pcDNA3.1V5-His Snk/Plk2 T239A

   Plasmid

   #22219

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, threonine …

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pEGFPC1-GluR1Ctail

   Plasmid

   #32441

   Depositor

   Robert Malinow

   Article

   Rumpel et al Science. 2005 Apr 1;308(5718):83-8. Epub

   Insert

   GluR1 (GRIA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains the last C-terminal 81 amino acids of th…

   Promoter

   CMV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-EIF3S3

   Plasmid

   #22545

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   EIF3S3(1-348aa) (EIF3H Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Tags

   T120>A

   Expression

   Bacterial

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJM189A

   Plasmid

   #218135

   Purpose

   For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD0
   

   Tags

   rpoZ

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Incoherent feedforward (IFF) plasmid

   Plasmid

   #169748

   Purpose

   Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-mito-eqFP650

   Plasmid

   #174179

   Purpose

   Express eqFP650 with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   eqFP650 with mitochondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-RAB-EKARev(T/A)

   Plasmid

   #118475

   Purpose

   Negative-control mutant for RAB-EKARev biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ExRai-AktAR(T/A)

   Plasmid

   #118463

   Purpose

   Negative-control mutant for ExRai-AktAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFX-EGFP-Sec61β

    Plasmid

    #174466

    Purpose

    Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. N1-mSin1.2-GFP

    Plasmid

    #72908

    Purpose

    GFP-tagged mSin1 (isoform 2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMito-miRFP718nano

    Plasmid

    #195754

    Purpose

    Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    Human cytochrome C oxidase subunit VIII-miRFP718nano
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MPRA Empty Vector

    Plasmid

    #204865

    Purpose

    Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

    Depositor

    Philipp Maass

    Article

    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES-Ap1g1-FKBP

    Plasmid

    #46945

    Purpose

    Co-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap1g1-FKBP
    

    Tags

    Mainly mouse Ap1g1 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbB8k-csg-amylase

    Plasmid

    #166859

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFX-mito-roGFP

    Plasmid

    #174181

    Purpose

    Express roGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    roGFP with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP(C3)-Nup153-C (880-1475)

    Plasmid

    #64317

    Purpose

    GFP-tagged mammalian expression of C-terminal domain of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    Nup153 (NUP153 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal subunit (880-1475)

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. CaV2.1 (pCR3.1)

    Plasmid

    #26573

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

    Plasmid

    #86871

    Purpose

    To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    SF3B6 (SF3B6 Human)
    

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    amino acids 7-29 deletion

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YFP-Rac1(N17)

    Plasmid

    #11395

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 mutated to Asparagine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGEX NBP1 (P#415)

    Plasmid

    #14658

    Depositor

    Matija Peterlin

    Article

    Lu et al Immunity. 1998 May . 8(5):647-56.

    Insert

    ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
    

    Expression

    Bacterial

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pGG-cTP-TurboID-GFP

    Plasmid

    #209403

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Tags

    GFP

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid

    #169490

    Purpose

    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFX-mito-EYFP

    Plasmid

    #174182

    Purpose

    Express EYFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EYFP with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQTEV-PSMD10

    Plasmid

    #31332

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PKG I alpha dNPS

    Plasmid

    #16391

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: delet…

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pCAG-cofilin S3A

    Plasmid

    #163604

    Purpose

    Expresses constitutively active cofilin in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Expression

    Mammalian

    Mutation

    S3A substitution to make cofilin constitutively a…

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHsSR beta deltaN Flag

    Plasmid

    #13731

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRPRB Human)
    

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    expresses residues 59-271 of Homo sapiens SRβ wit…

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRK5-HA-YFP-PRAS40

    Plasmid

    #73389

    Purpose

    Expresses HA and YFP tagged PRAS40

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    AKT1 Substrate 1 (Proline-Rich) (AKT1S1 Human)
    

    Tags

    HA and YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Available Since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. DNA-CARtcr-IRESpuro

    Plasmid

    #89346

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. YFP-Rac1(L61)

    Plasmid

    #11401

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glutamine 61 mutated to Leucine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pGG-GFP-PM-TurboID

    Plasmid

    #209402

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    cTP-3XHA-TurboID
    

    Tags

    GFP

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA4-Myc-eIF3f

    Plasmid

    #35975

    Depositor

    Susana Valente

    Article

    Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

    Insert

    eukaryotic translation initiation factor 3, subunit F (EIF3F Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RIPR-bio-His

    Plasmid

    #50824

    Purpose

    Expresses enzymatically monobiotinylated full length RIPR

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RIPR
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2-Flag-TTGZFP-FokI-DD

    Plasmid

    #18755

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge