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FLAG.PKCzeta.T410A
Plasmid#10801DepositorInsertPKC zeta (Prkcz Rat)
UseTagsFLAGExpressionMammalianMutationActivation loop inactive: T410A (Thr 410 to Ala)PromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-LC3
Plasmid#184900PurposeStable expression of HaloTag7-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE
Plasmid#104492PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dynamin-I-K44A-6His/Flag
Plasmid#32696DepositorInsertDynamin I (Dnm1 Rat)
UseTagsFlag and His6ExpressionMammalianMutationK44APromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pORANGE mEos3.2-Camk2a KI
Plasmid#131493PurposeEndogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR2Q024y
Plasmid#101022Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromotercmVAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP3bright
Plasmid#74423PurposeExpress fluorescent calcium indicator GCaMP3bright in mammalian cellsDepositorInsertGCaMP3bright
UseTagsExpressionMammalianMutationD392APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST1374-scFv-APOBEC-UGI-GB1
Plasmid#113029PurposeExpresses scFv-rAPOBEC1-UGI in mammalian cellsDepositorInsertscFv-rAPOBEC1-UGI-GB1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBK-VRER-BE3
Plasmid#85173PurposeExpresses VRER-BE3 in mammalian cellsDepositorInsertVRER-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
sh-SRF
Plasmid#100797Purposeexpression of shRNA targeting SRFDepositorInsertrat SRF
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218864PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI
Plasmid#196223PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 WT IRES mRuby3
Plasmid#170639PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 D303G IRES mRuby3
Plasmid#170640PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationD303GPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 D365E IRES mRuby3
Plasmid#170641PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationD365EPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D303G
Plasmid#170646PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD303GPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D365E
Plasmid#170649PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD365EPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) I367T
Plasmid#170652PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationI367TPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3(1-265)
Plasmid#100573PurposeExpresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.DepositorInsertStx3-1-265 (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain, residues 4-264 present.PromotertacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma8
Plasmid#165496PurposeGreen fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Jag1-HA pIRES
Plasmid#17336DepositorInsertJagged1 (Jag1 Rat)
UseTagsHA X3ExpressionMammalianMutationA silent C->G mutation at 3237PromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TrkB
Plasmid#24088DepositorInsertTrkB (Ntrk2 Rat)
UseTagsExpressionMammalianMutationR133IPromoterAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-F5A
Plasmid#8986DepositorInsertS6K1 (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationF5APromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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FLAG.PKCzeta
Plasmid#10799DepositorInsertPKC zeta (Prkcz Rat)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only
