We narrowed to 14,581 results for: pET

1. pET22HT-PP7delFG

   Plasmid

   #31863

   Depositor

   Robert Singer

   Article

   Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

   Insert

   PP7delFG Coat Protein
   

   Use

   Tags

   His6-tag and TEV site

   Expression

   Mutation

   deleted FG loop

   Promoter

   Available since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDRF1-GW ymYPET

   Plasmid

   #118455

   Purpose

   Overexpression of ymYPET

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YPET A206K, F208S, E232L, N235D

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RalGDS-YPet

   Plasmid

   #134929

   Purpose

   Fluorescently tagged affinity reagent

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   RaIGDS RBD, YPet
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-clbO

   Plasmid

   #114153

   Purpose

   Expresses ClbO

   Depositor

   Emily Balskus

   Article

   Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

   Insert

   clbO
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_6xHis-PARP1delBRCT

   Plasmid

   #173938

   Purpose

   Bacterial expression of PARP1 lacking BRCT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delBRCT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 374-520; V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETNb-nALFA

   Plasmid

   #159988

   Purpose

   For the protein expression with ALFA tag at N-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET26b_AT118-L-V5-His

   Plasmid

   #222848

   Purpose

   E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-Flavidin

   Plasmid

   #89881

   Purpose

   Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
   

   Use

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K80R, K121R, K132R, K134R

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET_RFP-SZ2

   Plasmid

   #124933

   Purpose

   Expression of RFP-SZ2

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RFP-SYNZIP2
   

   Use

   Tags

   His and mCherry

   Expression

   Bacterial

   Mutation

   SZ2 domain

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYpet-PBD

    Plasmid

    #22781

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-clbK

    Plasmid

    #114152

    Purpose

    Expresses ClbK

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbK
    

    Use

    Tags

    6xhis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET29b-clbI

    Plasmid

    #114151

    Purpose

    Expresses ClbI

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29b-clbF

    Plasmid

    #114148

    Purpose

    Expresses ClbF-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-clbC

    Plasmid

    #114145

    Purpose

    Expresses ClbC-nHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbC
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29b-clbE

    Plasmid

    #114147

    Purpose

    Expresses ClbE-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbE
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b-clbD

    Plasmid

    #114146

    Purpose

    Expresses ClbD-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET29b-clbG

    Plasmid

    #114150

    Purpose

    Expresses ClbG

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET29b-clbBpks

    Plasmid

    #114154

    Purpose

    Expresses the PKS module of ClbB

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Only contains the PKS module of ClbB

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-clbH-A1

    Plasmid

    #114149

    Purpose

    Expresses ClbH-A1

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbH-A1
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Contains only the A1 domain of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET26b-Granzyme A (S-A)

    Plasmid

    #8825

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A inactive (GZMA Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    changed Serine 184 to Alanine

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pET28_6xHis-PARP1delCAT

    Plasmid

    #173942

    Purpose

    Bacterial expression of PARP1 lacking CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delCAT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 661-1014

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b/bPanK

    Plasmid

    #73619

    Purpose

    Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

    Depositor

    T. J. Kappock, Suzanne Jackowski

    Article

    Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

    Insert

    pantothenate kinase (coaA Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    relative to MG1655 sequence, changes glutamate-5 …

    Promoter

    T7

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGlnA-Ypet

    Plasmid

    #98278

    Purpose

    mammalian expression of GlnA-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    GlnA
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-3c-rhVTN_FL

    Plasmid

    #30223

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 Apr 10. ():.

    Insert

    rhVitronectin without deletions (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No deletions

    Promoter

    Available since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET47b-T7-AsCas12f-WT

    Plasmid

    #204640

    Purpose

    Expression of wild-type AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YPet-pBAD

    Plasmid

    #54860

    Purpose

    Localization: Protein Expression Vector , Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Type

    Empty backbone

    Use

    Tags

    6xHis and YPet

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pET24_NusA-Tus

    Plasmid

    #165959

    Purpose

    Expression of soluble E. coli Tus protein

    Depositor

    Keith Pardee

    Article

    Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

    Insert

    Tus
    

    Use

    Tags

    His-NusA-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28_6xHis-PARP1delWGR

    Plasmid

    #173940

    Purpose

    Bacterial expression of PARP1 lacking WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETcLIC-GFP

    Plasmid

    #183514

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21a.Tat(48-57)-I-SceI

    Plasmid

    #165041

    Purpose

    Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    I-SceI
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCyPet-Rac1(WT)

    Plasmid

    #22782

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (wt) (RAC1 Human)
    

    Use

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pET-BoNT/E

    Plasmid

    #168020

    Purpose

    Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    BoNT/E-LC(1-411)-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET48 AtGUN1-PS

    Plasmid

    #136358

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pET32a-hBTC

    Plasmid

    #199230

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Betacellulin (BTC Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNup133-Ypet

    Plasmid

    #98283

    Purpose

    mammalian expression of Nup133-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup133 (NUP133 Human)
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-Cut-P(11)4

    Plasmid

    #185785

    Purpose

    Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Cut-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge