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Showing: 901 - 950 of 2333 results

  14. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Michael Ward
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110492-plasmid-map-sequence-id-207872

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  20. lentiCRISPR - CTRL sgRNA

    Plasmid
    #70662
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    non-targeting sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70662-plasmid-map-sequence-id-162511

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  22. lentiCRISPR - AAVS1 sgRNA

    Plasmid
    #70661
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against AAVS1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pCoofy50

    Plasmid
    #55189
    Purpose
    Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    8x His, FLAG, HA, PreScission site (3C protease s…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55189-plasmid-map-sequence-id-85571

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Showing: 901 - 950 of 2333 results