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We narrowed to 1,308 results for: grna cloning vector

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Showing: 901 - 950 of 1308 results

  2. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  3. pGG426_UV5

    Plasmid
    #165608
    Purpose
    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1135V, S1136R, D1332K and R1333G mutations in Sp…
    Promoter
    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165608-plasmid-map-sequence-id-330965

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  22. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Article
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  24. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid
    #124299
    Purpose
    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    UBE2G1 (UBE2G1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124299-plasmid-map-sequence-id-244604

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  25. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid
    #124300
    Purpose
    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    SENP8 (SENP8 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124300-plasmid-map-sequence-id-242478

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  36. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  37. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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Showing: 901 - 950 of 1308 results