We narrowed to 6,685 results for: tac

1. SAE2-mGarnet

   Plasmid

   #175240

   Purpose

   Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBA2 (UBA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

   Plasmid

   #135455

   Purpose

   Bacterial expression of uTEV1Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, S153N mutation…

   Promoter

   tac promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-4T1-RhoA G17A

   Plasmid

   #69357

   Purpose

   expression of nucleotide free RhoA in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G17A

   Promoter

   tac

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pLKO.1-scrCdh20.1 GFP

   Plasmid

   #209106

   Purpose

   GFP expressing scramble of shRNA targeting Cdh20

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCdh20.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1-hPREP

   Plasmid

   #179387

   Purpose

   Express human prolyl oligopeptidase (tagged) in bacterial cells

   Depositor

   Christian Gruber

   Article

   Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

   Insert

   human prolyl oligopeptidase (PREP Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

   Plasmid

   #135454

   Purpose

   Bacterial expression of TEVΔ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability. Truncated form…

   Promoter

   tac promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-6P-1 Stu2 1-306

   Plasmid

   #38315

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 918 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-KG-CAND1

   Plasmid

   #19949

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-HA-GlpG

    Plasmid

    #155135

    Purpose

    Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

    Plasmid

    #222989

    Purpose

    Bacterial expression of iGeoCas9(G) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(G)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX2TK-Osh2-PH

    Plasmid

    #71370

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-4T1-Cdc42 G15A

    Plasmid

    #69356

    Purpose

    expression of nucleotide free Cdc42 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGEX2TK-Num1-PH

    Plasmid

    #71369

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Num1-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa2563-2692

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGEX2TK-Skm1-PH

    Plasmid

    #71371

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Skm1-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa1-132

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSub-MBP-GFP(2M-IKQE)

    Plasmid

    #185757

    Purpose

    Bacterial expression of MBP-GFP(2M IKQE)

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    GFP(2M-IKQE)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST-N1IC

    Plasmid

    #47612

    Purpose

    bacterial expression of GST-myc-Notch1 IC

    Depositor

    Urban Lendahl

    Article

    Beatus et al Development. 1999 Sep;126(17):3925-35.

    Insert

    Notch1 intracellular domain (Notch1 Mouse)
    

    Use

    Tags

    GST and myc

    Expression

    Bacterial

    Mutation

    contains amino acids 1753-2531

    Promoter

    tac

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGEX 4T-1-PPARgamma2

    Plasmid

    #78773

    Purpose

    To purify GST-tagged recombinant protein

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

21. GST eIF2beta

    Plasmid

    #45900

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 (Eif2s2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

22. GST-CLCb

    Plasmid

    #47404

    Depositor

    Peter McPherson

    Article

    Legendre-Guillemin et al J Biol Chem. 2005 Feb 18;280(7):6101-8.

    Insert

    CLCb
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

23. GST-PRMT9 G260E mutant

    Plasmid

    #67610

    Purpose

    bacterial expression of GST-PRMT9 G260E Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G260E double E loop mutant

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

24. GST-PRMT9 D258G mutant

    Plasmid

    #67609

    Purpose

    bacterial expression of GST-PRMT9 D258G Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D258G double E loop mutant

    Promoter

    tac

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pFM111

    Plasmid

    #40157

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-1N:FLAG (par-1 Nematode)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Contains aa 1-964

    Promoter

    tac

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pFM112

    Plasmid

    #40158

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-1C:FLAG (par-1 Nematode)
    

    Use

    Tags

    GST and modified Flag

    Expression

    Bacterial

    Mutation

    Contains aa 966-1192

    Promoter

    tac

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

27. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

    Plasmid

    #222988

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1-shCdh2.1 GFP

    Plasmid

    #209099

    Purpose

    GFP expressing shRNA targeting Cdh2

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1-shCdh20.1 GFP

    Plasmid

    #209105

    Purpose

    GFP expressing shRNA targeting Cdh20

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh20.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Dhh1 25-425-EGFP

    Plasmid

    #201430

    Purpose

    Bacterial protein expression of Dhh1 25-425

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 25-425 only

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Dcp2 301-970-EGFP

    Plasmid

    #201429

    Purpose

    Bacterial protein expression of Dcp2 301-970

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 301-970 only

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Dhh1 426-506-EGFP

    Plasmid

    #201431

    Purpose

    Bacterial protein expression of Dhh1 426-506

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 426-506 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Dcp1 D82-129-EGFP

    Plasmid

    #201426

    Purpose

    Bacterial protein expression of Dcp1 del82-129

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp1 (DCP1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    deletion of aa 82-129

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Edc3 1-66-EGFP

    Plasmid

    #201432

    Purpose

    Bacterial protein expression of Edc3 1-66

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Edc3 (EDC3 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 1-66 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Dcp1 82-129-EGFP

    Plasmid

    #201427

    Purpose

    Bacterial protein expression of Dcp1 82-129

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp1 (DCP1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 82-129 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Pat1 1-240-EGFP

    Plasmid

    #201435

    Purpose

    Bacterial protein expression of Pat1 1-240

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Pat1 (PAT1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 1-240 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Edc3 67-282-EGFP

    Plasmid

    #201433

    Purpose

    Bacterial protein expression of Edc3 67-282

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Edc3 (EDC3 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 67-282 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Pat1 241-422-EGFP

    Plasmid

    #201436

    Purpose

    Bacterial protein expression of Pat1 241-422

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Pat1 (PAT1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 241-422 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Dcp2 1-300-EGFP

    Plasmid

    #201428

    Purpose

    Bacterial protein expression of Dcp2 1-300

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 1-300 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Pat1 423-796-EGFP

    Plasmid

    #201437

    Purpose

    Bacterial protein expression of Pat1 423-796

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Pat1 (PAT1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 423-796 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Edc3 283-551-EGFP

    Plasmid

    #201434

    Purpose

    Bacterial protein expression of Edc3 283-551

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Edc3 (EDC3 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 283-551 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX-6P-1 Monosiga brevicollis CBS-pair domain

    Plasmid

    #166435

    Purpose

    Express pGEX-6P-1 Monosiga brevicollis CBS-pair domain

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Monosiga brevicollis CBS-pair domain
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac, LacI, Amp

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGEX-6P-1 Naegleria fowleri CBS-pair domain

    Plasmid

    #166437

    Purpose

    Express pGEX-6P-1 Naegleria fowleri CBS-pair domain

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Naegleria fowleri CBS-pair domain
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac, LacI, Amp

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGEX-6P-1 Toxoplasma gondii CBS-pair domain

    Plasmid

    #166438

    Purpose

    Express pGEX-6P-1 Toxoplasma gondii CBS-pair domain

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Toxoplasma gondii CBS-pair domain
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac, LacI, Amp

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSub-Asp6-MBP-HRas

    Plasmid

    #185758

    Purpose

    Bacterial expression of Asp6-MBP-HRas

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    Asp6-MBP-HRas
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    6x Asp added to the N terminus of MBP

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. FKBP-EGFP-RanGAP

    Plasmid

    #175236

    Purpose

    Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. EGFP-RanGAP

    Plasmid

    #175235

    Purpose

    Bacterial expression and purification, high affinity SUMOylation substrate

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGEX-4T-1 dATG1 (1188)

    Plasmid

    #66918

    Purpose

    bacterial expression of dATG1

    Depositor

    Michael Karin, Jun Hee Lee

    Article

    Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

    Insert

    ATG1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. GST-prRDH-CTS

    Plasmid

    #86069

    Purpose

    A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Retinol dehydrogenase (RDH8 Bovine)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    the insert is 297-312 of RDH8 (Bos taurus)

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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