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NLS-HA-2xMCP-Ezh2

Plasmid

#126590

Purpose

Expresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-Ezh2 (Ezh2 Mouse, Synthetic)

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CATULIN

Plasmid

#118979

Purpose

Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Alpha Catulin (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR/U6 HDAC5 shRNA

Plasmid

#32222

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against mouse HDAC5 (Hdac5 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
M71-IRES-tauGFP LTNL TV

Plasmid

#15639

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M71-IRES-tauGFP LTNL TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pCWXPGR-pTF-NT3

Plasmid

#114279

Purpose

TET-inducible expression of the Neurotrophin 3 protein

Depositor

Patrick Salmon

Insert

Neurotrophin 3 (Ntf3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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IRE1bpcDNA3

Plasmid

#21879

Depositor

David Ron

Insert

IRE1b (Ern2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pISH-OCAM

Plasmid

#136767

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Ncam2

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Ncam2 (Ncam2 Mouse)

Use

In situ probe

Tags

Expression

Mutation

ISH Probe insert

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-B4

Plasmid

#31708

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-92a

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-19a-20-19b

Plasmid

#24827

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir19a-mir20-mir19b

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

Plasmid

#124870

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

Plasmid

#62977

Purpose

Expresses myc-tagged mouse βTrCP residues 241-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

codes for residues 241-569

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pET21a-mAIM2-deltaPYD

Plasmid

#51535

Purpose

Express His6-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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DAXX-myc

Plasmid

#1851

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP-Syx2

Plasmid

#35705

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pX458-sgRNA_Ago2_3

Plasmid

#73531

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shREN

Plasmid

#105585

Purpose

Dox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMyt1GFPicre-Knock-In

Plasmid

#22657

Depositor

Lynn Hudson

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Use

Targeting vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Use

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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p5xFbx15 (-544/-527)-TK-Luc

Plasmid

#15686

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Tags

Expression

Mutation

Wild-type

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-FKBP-mCherry-Vav2cat

Plasmid

#178865

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to FKBP-mCherry

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mCherry-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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LZRS ERTm RasN17

Plasmid

#21198

Depositor

Paul Khavari

Article

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

HRas (Esr1, HRAS Mouse, Human)

Use

Retroviral

Tags

Expression

Mutation

S17N ERTm bx3

Promoter

Available since

June 16, 2010

Availability

Academic Institutions and Nonprofits only
AIRAP 8: pCEFL.PSMB2.GST

Plasmid

#21798

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMB2 (Psmb2 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V7-CD2

Plasmid

#199186

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V7-CD2

Use

AAV

Tags

Expression

Mammalian

Mutation

EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V8-CD2

Plasmid

#199187

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V8-CD2

Use

AAV

Tags

Expression

Mutation

EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

Plasmid

#173761

Purpose

Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

Depositor

Peter Wright

Article

Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-PSD-95 [K28/74R]

Plasmid

#177439

Purpose

Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/74.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-PSD-95 (Homo sapiens) recombinant mouse monoclonal antibody (DLG4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mMcl-1

Plasmid

#32980

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pCWXPGR-pTF-NeuroD1

Plasmid

#114278

Purpose

TET-inducible expression of the NeuroD1 protein

Depositor

Patrick Salmon

Insert

NeuroD1 (Neurod1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean-H3.3-N-14

Plasmid

#55377

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

H3.3 (H3f3b Mouse)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-Emx2

Plasmid

#74346

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

empty spiracles homeobox 2 (Emx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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