Addgene

We narrowed to 26,420 results for: CAL

Showing: 7561 - 7600 of 26420 results

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KBB466

Plasmid

#185099

Purpose

NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1DNLS2-GFP

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB467

Plasmid

#185100

Purpose

NMA111-GFP with NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS2-GFP

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S(-31)-M30_dT7term

Plasmid

#112253

Purpose

Expresses the S(-31)-M30 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S*5-M5_dT7term

Plasmid

#112254

Purpose

Expresses the S*5-M5 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S*5-M5 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-EnR

Plasmid

#185497

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Yan et al Dev Biol. 2009 May 1;329(1):80-95. doi:

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

fusion of winged helix domain with EnR domain

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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miniCMV-mCerulean-tDeg

Plasmid

#185401

Purpose

mCerulean-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

mCerulean-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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NK85

Plasmid

#176611

Purpose

To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

FCERG

Use

Lentiviral

Tags

EGFP and HA, SNAP-tag

Expression

Mammalian

Mutation

C25A

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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NK83

Plasmid

#176610

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

NeonGreen-tSH2 Syk (Syk Synthetic, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/eCFP-C

Plasmid

#183156

Purpose

Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179029

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179028

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-TARG1

Plasmid

#172577

Purpose

For gateway cloning of C-terminal tagged TARG1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-mtIF3-Dendra2

Plasmid

#182371

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

Tags

Pal-Dendra2

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3137

Plasmid

#179617

Purpose

Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

GCaMP6

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Punc-4

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T-Y10AL15A_D

Plasmid

#146153

Purpose

Insect Expression of Dm4E-T-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-Y10AL15A (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_D

Plasmid

#146150

Purpose

Insect Expression of Dm4E-T

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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E6SCX5

Plasmid

#163275

Purpose

Inducible expression of UniProt identifier E6SCX5

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

E6SCX5

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCm No carrier NikJC199U

Plasmid

#174361

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Use

Tags

8xHis and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MAPRE1-Halo

Plasmid

#175345

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MAPRE1 (MAPRE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA43

Plasmid

#172944

Purpose

pQE30 encoding Wzz mutant i191

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i191 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA47

Plasmid

#172947

Purpose

pQE30 encoding Wzz mutant i255

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i255 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA49

Plasmid

#172948

Purpose

pQE30 encoding Wzz mutant i81

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i81 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA42

Plasmid

#172943

Purpose

pQE30 encoding Wzz mutant i290

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i290 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA52

Plasmid

#172938

Purpose

pQE30 encoding Wzz mutant i138

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i138 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA41

Plasmid

#172935

Purpose

pQE30 encoding Wzz mutant i231

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i231 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA56

Plasmid

#172951

Purpose

pQE30 encoding Wzz mutant i66

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i66 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA46

Plasmid

#172946

Purpose

pQE30 encoding Wzz mutant i279

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i279 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA51

Plasmid

#172949

Purpose

pQE30 encoding Wzz mutant i247

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i247 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA40

Plasmid

#172942

Purpose

pQE30 encoding Wzz mutant i163

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i163 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA39

Plasmid

#172940

Purpose

pQE30 encoding Wzz mutant i128

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i128 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA44

Plasmid

#172945

Purpose

pQE30 encoding Wzz mutant i161

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i161 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA53

Plasmid

#172939

Purpose

pQE30 encoding Wzz mutant i80

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i80 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
JGI_018311

Plasmid

#158833

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

FAM1_3631

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
JGI_018387

Plasmid

#158871

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Methyloceanibacter_5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7561 - 7600 of 26420 results

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