We narrowed to 29,536 results for: REP

1. SGEP

   Plasmid

   #111170

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-1x

   Plasmid

   #172281

   Purpose

   Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-1x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAP2

   Plasmid

   #73211

   Purpose

   express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   AAP2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. sUPRa

   Plasmid

   #223242

   Purpose

   sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

   Depositor

   Colin Akerman

   Article

   Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

   Insert

   sUPRa
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Custom

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRubiG-T2A-Cas9

   Plasmid

   #75348

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Green Lindoblum

   Plasmid

   #163113

   Purpose

   Expresses biosensor for lactate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Lindolum
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_DNAseI

    Plasmid

    #182408

    Purpose

    Bacterial expression of DNaseI

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-wtSrc

    Plasmid

    #140295

    Purpose

    Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST40-2XHA-wtSrc

    Plasmid

    #140294

    Purpose

    Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-mPKR-FLAG-IG

    Plasmid

    #101792

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse PKR

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Pkr (Eif2ak2 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-4031NES

    Plasmid

    #105241

    Purpose

    A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    AMPK-EV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3xFLAG-CUL4A-pCMV7.1

    Plasmid

    #155022

    Purpose

    N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 4A (CUL4A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAD-KPS12-Pman

    Plasmid

    #133832

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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