We narrowed to 22,937 results for: REP
-
Plasmid#159537PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 with two guide RNA cassettes
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
Plasmid#113862PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc
UseLentiviralExpressionMammalianAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046785
Plasmid#145684PurposeBacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDT-sgRNA
Plasmid#138271PurposeDual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)DepositorInsertsgBG, sgTS
UseCRISPRExpressionMammalianAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
458 p5E-uasE1b
Plasmid#26026DepositorInsertE1B basal promoter + UAS repeats
UseGateway CloningAvailable SinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)
Plasmid#175574PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-gRNA
Plasmid#158973PurposeA human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.DepositorInsertgRNA3
ExpressionMammalianPromoterU6Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pγsat
Plasmid#39238DepositorInsert8 copies of the 234bp mouse γ-satellite repeat
UseCloning vectorAvailable SinceJan. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCVhygro-F-G9adSET
Plasmid#41722PurposeRetroviral expression of human G9a mutant in mammalian cellsDepositorAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-mtdT-3XHA
Plasmid#24354DepositorInsertmtDT-3XHA
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway CloningAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralPromotermCMVAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMYH9-WT-V5
Plasmid#183512PurposeGateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.DepositorAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145
Plasmid#111731PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ145 (polyQ repeat)PromoterCMV and P10Available SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN3-MGA
Plasmid#107715PurposeExpression plasmid for 3xFLAG-tagged MGADepositorAvailable SinceApril 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-FLAG-Luc-N
Plasmid#58790PurposeExpress LucN alone in mammalian cellsDepositorInsertfirefly Luciferase 1-398
TagsFLAGExpressionMammalianMutationT576C,T591C to destroy MfeI and EcoRI sitesPromoterCMVAvailable SinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
YUC4:RUBY
Plasmid#160907PurposeRUBY under the control of YUC4 promoter/tissue specific marker for gene expressionDepositorInsertRUBY and YUC4 promoter
ExpressionPlantPromoterYUC4Available SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQUAS-GG
Plasmid#24336DepositorInsert5xQUAS/GFP split with an intron (GG)
ExpressionMammalianAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTE-10M0X
Plasmid#20318DepositorInsertPimyc-tetR#10
ExpressionBacterialAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only