160,990 results

1. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

   Plasmid

   #228364

   Purpose

   mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Enascas12a nuclease and en…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS2513·PHP

   Plasmid

   #122590

   Purpose

   Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

   Depositor

   Pablo Ivan Nikel

   Article

   Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

   Insert

   phIorine2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pem7

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPR37L1-Tango

   Plasmid

   #66356

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR37L1 (GPR37L1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pMKO.1 GFP

   Plasmid

   #10676

   Purpose

   Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

   Plasmid

   #174876

   Purpose

   PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   RNAPII Ser2ph mintbody-SNAP
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-FLT3-V5/HIS

   Plasmid

   #201985

   Purpose

   expression of human FLT3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   FLT3 receptor tyrosine kinase (FLT3 Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Green Glifon600

   Plasmid

   #126207

   Purpose

   green fluorescent glucose sensor for mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

   Insert

   Green Glifon600
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Promoterless

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP Vinculin

   Plasmid

   #50513

   Depositor

   Kenneth Yamada

   Insert

   Vinculin (VCL Chicken)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. dSaCas9-VPR

    Plasmid

    #205251

    Purpose

    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSaCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. XPO6-EGFP

    Plasmid

    #210371

    Purpose

    Expresses XPO6 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    XPO6 (XPO6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GABRA1-IRES2-EGFP

    Plasmid

    #170820

    Purpose

    human GABAA receptors alpha1 subunit and IRES2-EGFP

    Depositor

    Tingwei Mu

    Article

    Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

    Insert

    Human GABAA receptor alpha1 subunit (GABRA1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-Matr3 delZnF1

    Plasmid

    #32883

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    deleted amino acids 283-322

    Promoter

    CMV

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-Matr3 delZnF2

    Plasmid

    #32884

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    deleted amino acids 793-833

    Promoter

    CMV

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pBABE zeo PPP2CA Wt

    Plasmid

    #10689

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

    Plasmid

    #169328

    Purpose

    Expression of HaloTag9 located at the microtubules of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag9 (CEP41 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-V5-SBP-C1-HsUpf1-siRNAres_P

    Plasmid

    #147196

    Purpose

    Mammalian Expression of HsUpf1-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    HsUpf1-siRNAres (UPF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 non silent mutations A69S, E167G, K282R, A442G…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIP-SFFV-EGFP-FLAG-cGAS

    Plasmid

    #127661

    Purpose

    Over-express EGFP-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP-Flag

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3-V5-Lin28A

    Plasmid

    #51387

    Depositor

    Narry Kim

    Article

    Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

    Insert

    Lin28A (LIN28A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3 proHB-EGF NC

    Plasmid

    #11605

    Depositor

    Rosalyn Adam

    Article

    Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pMuLE ENTR MCS R4-R3

    Plasmid

    #62086

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. eGFP-hCOL1A1

    Plasmid

    #140110

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag, K20…

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCitrine-Lifeact-7

    Plasmid

    #54733

    Purpose

    Localization: Actin, Excitation: 516, Emission: 529

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCitrine-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid

    #125256

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2(H134R)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-forward

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. EMTB-mNeonGreen

    Plasmid

    #137802

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Ensconsin microtubule binding domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_6xHis-PARP1delZn2

    Plasmid

    #173935

    Purpose

    Bacterial expression of PARP1 lacking Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 (V762A)

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGM227

    Plasmid

    #220182

    Purpose

    Expresses exogenous wild-type TOMM70A

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TOMM70A (TOMM70 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge