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173,764 results

Showing: 5731 - 5760 of 173764 results

  7. pER-rxRFP1.4

    Plasmid
    #67842
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.4, endoplasmic reticulum localized
    Depositor
    Huiwang Ai
    Article
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Insert
    rxRFP1.4
    Tags
    ER localization, ER retention, and His Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    67842-plasmid-map-sequence-id-244285

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  10. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  15. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  19. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid
    #123235
    Purpose
    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123235-plasmid-map-sequence-id-378779

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  20. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    Monomer Red Fluorescent Protein
    Expression
    Mammalian
    Available Since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    13032-plasmid-map-sequence-id-211651

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  22. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

    Plasmid
    #183607
    Purpose
    Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    LaG17 GFP nanobody SynNotch tTA
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Promoter
    Mouse Pgk1
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183607-plasmid-map-sequence-id-361593

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  30. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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Showing: 5731 - 5760 of 173764 results