162,590 results

1. pGP-CMV-NES-jRCaMP1b

   Plasmid

   #63136

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1b
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA

   Plasmid

   #158390

   Purpose

   Inducible Bxb1 integrase for mammalian cells

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   Bxb1-ecDHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SF-SON IRES Tomato

   Plasmid

   #214403

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen_taq

   Plasmid

   #153315

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   Drew Endy

   Insert

   Taq polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124429

   Purpose

   Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(TEV cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. R26-pCAG-Ceru-10unit-BGHpA

   Plasmid

   #158387

   Purpose

   10-unit intMEMOIR recording array for mammalian cells

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   intMEMOIR 10-unit array
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-Blasticidin

   Plasmid

   #75085

   Purpose

   retrovirus-mediated gene transfer into mammalian cells

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGS-21a his6-GST-WTPhi29DNAP

   Plasmid

   #166863

   Purpose

   To produce a dual affinity-tagged Phi29 DNA polymerase

   Depositor

   Stephen Quake, Paul Blainey

   Article

   Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

   Insert

   Phi29 Polymerase codon-optimized for overexpression in E. coli
   

   Use

   Tags

   GST and his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EB3N-VVDfast-mCl3-iLID

   Plasmid

   #190165

   Purpose

   Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   EB3N-VVDfast-mClover3-iLID
   

   Use

   Tags

   -VVDfast-mClover3-iLID

   Expression

   Mammalian

   Mutation

   1-189 aa from EB3

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

    Plasmid

    #118593

    Purpose

    Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha/TRE3G

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Luc

    Plasmid

    #45968

    Depositor

    Ralf Kuehn

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSyn.iGluSnFr.WPRE.SV40

    Plasmid

    #98929

    Purpose

    AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    iGluSnFr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJJB1439

    Plasmid

    #218589

    Purpose

    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Erg20(F96W, N127W)

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-TetOn-mCherry-OAZ1_FS-YFP

    Plasmid

    #232356

    Purpose

    Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

    Depositor

    Ankur Jain

    Article

    Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

    Insert

    OAZ1 derived polyamine sensing module (OAZ1 Human)
    

    Use

    Lentiviral

    Tags

    eYFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCLXEBR-pTF-hM3Dq

    Plasmid

    #114283

    Purpose

    TET-inducible expression of the hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PRExpress-Bxb1-hsp70pA

    Plasmid

    #158391

    Purpose

    heat shock inducible Bxb1 integrase for drosophila melanogaster

    Depositor

    Michael Elowitz

    Article

    Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

    Insert

    Bxb1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIND-HA-ADH

    Plasmid

    #11991

    Depositor

    Steven Johnson

    Insert

    aldehyde dehydrogenase (ALDH1A1 Human)
    

    Use

    Ecdysone inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

22. CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

    Plasmid

    #127872

    Purpose

    Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Ntn4-Fc-His

    Plasmid

    #72106

    Purpose

    Expresses the entire Netrin 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn4 (Ntn4 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pATetO 6XHis-Phusion

    Plasmid

    #206210

    Purpose

    Inducible expression of DNA polymerase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    Phusion DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLminP_Luc2P_RE60

    Plasmid

    #90405

    Purpose

    STAT5 - Stat5 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SIE

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPRISM-TagRFP-cmlc2-eGFP

    Plasmid

    #117770

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAB213

    Plasmid

    #101677

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-Myc-IRES-RFP

    Plasmid

    #35395

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

    Inserts

    Myc (Myc Mouse)
    RFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

30. UAS-Ceru-10unit

    Plasmid

    #158389

    Purpose

    10-unit intMEMOIR recording array for Drosophila melanogaster

    Depositor

    Michael Elowitz

    Article

    Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

    Insert

    10unit recording array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge