We narrowed to 34,448 results for: CaS

1. pGEX4T3-GST-PolB(L301R/V303R/V306R)

   Plasmid

   #177137

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX4T3-GST-PolB(K206A/K244A/T304I)

   Plasmid

   #177134

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmxs_COX4

   Plasmid

   #177979

   Purpose

   Mammalian expression of COX4 cDNA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   COX4 (COX4I1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to COX4 sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSIN-CD45

   Plasmid

   #175943

   Purpose

   knockout mouse CD45

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   CD45 gRNA (Ptprc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGK-Ptrf-mKate2

   Plasmid

   #128765

   Purpose

   Fluorescent mKate reporter for Ptrf

   Depositor

   Deepak Srivastava

   Article

   Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

   Insert

   Ptrf promoter (Cavin1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ptrf

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCEP4-myc-HLA-A2-VN (G112P)

   Plasmid

   #135496

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   G112P

   Promoter

   CMV

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCEP4-myc-HLA-A2-VN (L156P)

   Plasmid

   #135497

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   L156P

   Promoter

   CMV

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

   Plasmid

   #136063

   Purpose

   Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Dd (H2-D1 Mouse)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Y84C/C121S/A139C

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCEP4-myc-HLA-B15-VN

   Plasmid

   #135514

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*15:01 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEP4-myc-HLA-B57-VN

    Plasmid

    #135515

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*57:1 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCEP4-myc-H2-Dd-VN

    Plasmid

    #135517

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Dd

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-myc-HLA-B8-VN

    Plasmid

    #135511

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*08:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEP4-myc-HLA-A2-VN (I52P)

    Plasmid

    #135486

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    I52P

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCEP4-myc-HLA-A2-VN (Y59P)

    Plasmid

    #135492

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Y59P

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-myc-HLA-A2-VN (D61P)

    Plasmid

    #135493

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    D61P

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-myc-HLA-A2-VN (V67P)

    Plasmid

    #135494

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    V67P

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-myc-HLA-A2-VN (Y84P)

    Plasmid

    #135495

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Y84P

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-myc-HLA-A2-VN (E166P)

    Plasmid

    #135498

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    E166P

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJC20cdc8.A18T

    Plasmid

    #99360

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJC20cdc8.E31K

    Plasmid

    #99361

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 31 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJC20cdc8.110

    Plasmid

    #99363

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine and Glutamic acid 31 to L…

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MoFGFR31c-ires neo

    Plasmid

    #86496

    Purpose

    mamalian cell expression of FGFR3-FGFR1 chimera

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR3-1 IIIc 15C7 (Fgfr1, Fgfr3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FGFR3-FGFR1 chimera

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MoFGFR31b-ires neo

    Plasmid

    #86495

    Purpose

    mamalian cell expression of FGFR3-FGFR1 chimera

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR31 IIIb 15C8 (Fgfr3, Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FGFR3-FGFR1 chimera

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RalA (G23V)(mature peptide)-pcw107-V5

    Plasmid

    #64643

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    G23V

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

25. RalA (G23V)(mature peptide)-pcw107

    Plasmid

    #64642

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G23V

    Promoter

    PGK

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

26. mSTIM1_1-250-CFP

    Plasmid

    #69552

    Purpose

    Expresses a STIM1_1-250 fragment mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    mSTIM1_1-250
    

    Use

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA5-F-Med26-NTD

    Plasmid

    #49255

    Purpose

    Mammalian expression of flag-tagged Med26-NTD

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD (aa 1-339)

    Promoter

    Available since

    Dec. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA5-F-Med26-R61A K62A

    Plasmid

    #49254

    Purpose

    Mammalian expression of flag-tagged human Med26 R61A K62A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R61A K62A

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pEMS1396

    Plasmid

    #29194

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple146 (NTSR1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEMS1174

    Plasmid

    #29088

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple240 (UGT8 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEMS1617

    Plasmid

    #29279

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple146 (NTSR1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades…

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pEMS1712

    Plasmid

    #29297

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple240 (UGT8 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pcDNA3.1mLCAD_K42Q_FLAG

    Plasmid

    #24487

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Gln

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pcDNA3.1mLCAD_K42A_FLAG

    Plasmid

    #24488

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Ala

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

35. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

    Plasmid

    #111398

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

    Plasmid

    #111399

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

    Plasmid

    #111400

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

    Plasmid

    #111451

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1

    Plasmid

    #111462

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/S536A/L540A, C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1

    Plasmid

    #111497

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/S536D/L540D, C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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41. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

    Plasmid

    #217306

    Purpose

    sgRNA mcherry + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGBScrispri Sham

    Plasmid

    #223201

    Purpose

    Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. SGEP-sh-Hs-PHGDH-1956

    Plasmid

    #188670

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. SGEP-sh-Hs-PHGDH-1883

    Plasmid

    #188669

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHEE-R-hyg9si

    Plasmid

    #222832

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHEE-R-hyg5A9si

    Plasmid

    #222835

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Banshee Runx1FL mCherry

    Plasmid

    #80157

    Purpose

    retroviral expression of Runx1Fl and mCherry

    Depositor

    Ellen Rothenberg

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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