We narrowed to 13,473 results for: Mpl

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pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold

Plasmid

#186981

Purpose

mammalian expression of Cas7-11 crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

mature DR guide

Expression

Mammalian

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot6L

Plasmid

#37369

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot6L (CNOT6L Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-neo VPS54-13Myc

Plasmid

#175095

Purpose

13Myc tagged VPS54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Gershlick et al Hum Mol Genet. 2019 May 1;28(9):1548-156

Insert

VPS54 (VPS54 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

Plasmid

#159853

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(PATC)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-MSL3L1

Plasmid

#29761

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MSL3L1 (MSL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Tags

SV40 NLS

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-RIP1

Plasmid

#44159

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

RIP1 (RIPK1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z29

Plasmid

#120072

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEF6 VPS52-V5-6His

Plasmid

#175096

Purpose

V5 tagged VPS52 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

VPS52 (VPS52 Human)

Tags

V5

Expression

Mammalian

Mutation

N666D- please see depositor comment

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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3TERM_Nos-35S

Plasmid

#136116

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos-35S (A. tumefaciens, CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-emerin-EGFP-polyA

Plasmid

#59747

Purpose

Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX

Plasmid

#166229

Purpose

Lentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

mCherry-CAAX

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKHR5

Plasmid

#74593

Purpose

an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette

Use

Cre/Lox; Zebrafish expression

Promoter

alpha-crystallin

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIIG

Plasmid

#186828

Purpose

Expresses FLAG-CaMKIIG in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2g (Camk2g Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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Col2.3deltaTK

Plasmid

#114273

Purpose

Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

Depositor

David Rowe

Article

Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

Insert

Col2.3 promoter + deltaTK + BGH polyA

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP-ELYS-polyA

Plasmid

#59746

Purpose

Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

EGFP-ELYS

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST TNRC6C

Plasmid

#19885

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pCk3_spacer

Plasmid

#136073

Purpose

Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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