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pLX_305_Ovalbumin

Plasmid

#184924

Purpose

Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Depositor

Arlene Sharpe

Publication

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

Ovalbumin (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Mutation

Our vector has an A188T mutation that does not im…

Promoter

PGK

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Publication

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-hSynI-V5-TurboID(N)-GPI

Plasmid

#163696

Purpose

Expresses n-terminal half of TurboID on surface of neurons

Depositor

Scott Soderling

Publication

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Publication

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Publication

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-sACE2(WT)-Fc(IgG1)

Plasmid

#145163

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Publication

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

Plasmid

#135391

Purpose

Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

Depositor

Fan Wang

Insert

TeLC-P2A-EYFP

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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CMV-mCherry-(F30-2xPepper)10

Plasmid

#129401

Purpose

mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-PIK3CA-H1047R

Plasmid

#116500

Purpose

Lentiviral expression of PIK3CA H1047R

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

H1047R

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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HA-Halotag-FRB-EX-nes_pLX304

Plasmid

#120913

Purpose

Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

HA-Halotag-FRB-EX-NES

Use

Lentiviral

Expression

Mammalian

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Publication

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJMP1039

Plasmid

#119239

Purpose

expresses Tn7 transposase

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

tnsABCD

Expression

Bacterial

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pmGFP-ADAR2

Plasmid

#117929

Purpose

ADAR2 expression plasmid.

Depositor

Kumiko Ui-Tei

Publication

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR2 (ADARB1 Human)

Tags

myc-EGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pC1-SypHer3s

Plasmid

#108118

Purpose

Ratiometric pH probe with enhanced brightness for mammalian cells expression.

Depositor

Vsevolod Belousov

Publication

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SypHer3s

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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