We narrowed to 14,009 results for: flag

1. pLenti-PGK-flag-RAP1B-N17-neo

   Plasmid

   #156176

   Purpose

   Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

   Plasmid

   #13617

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 65, Thr 69 and Ser70 to Asp

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)

   Plasmid

   #74505

   Purpose

   Overexpression of CSK SH3-SH2 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

   Insert

   CSK (CSK Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   SH3-SH2 domains

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pPB CAG Gfi1:FLAG-IRES-EGFP

   Plasmid

   #153945

   Purpose

   piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-BLAST flag-CAD S1396A

   Plasmid

   #188126

   Purpose

   Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

   Plasmid

   #120391

   Purpose

   pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

   Type

   Empty backbone

   Use

   AAV

   Tags

   mCherry.3XFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

   Plasmid

   #29446

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 520-1556 (a.a.)

   Promoter

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

   Plasmid

   #19845

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Promoter

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pRK5 FLAG membrane-bound lipin-1 (TMD)

   Plasmid

   #120277

   Purpose

   Expression of ER/Golgi-tethered lipin-1 in mammalian cells

   Depositor

   Sirio Dupont

   Article

   Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

   Insert

   Lipin-1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG and in-frame fusion with the TMD Golgi-local…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

    Plasmid

    #127775

    Purpose

    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox10 (SOX10 Chicken)
    

    Use

    Enhancer reporter construct

    Tags

    Avi

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid

    #49065

    Purpose

    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA Flag-RARalpha (T181A,S445A,S461A)

    Plasmid

    #35556

    Depositor

    Harish Srinivas, Jonathan Kurie

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    T181A, S445A, S461A

    Promoter

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

    Plasmid

    #166809

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB

    Plasmid

    #131350

    Purpose

    Expresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-GyrB (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

    Plasmid

    #166801

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    S730A

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

    Plasmid

    #29439

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 267-1261 (a.a.) with Q1116K mutation

    Promoter

    Available Since

    Aug. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid

    #121751

    Purpose

    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FLAG epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

    Plasmid

    #13616

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65, Thr 69 and Ser 70 to Alanine

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

    Plasmid

    #29437

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 267-1261 (a.a.) E653Q

    Promoter

    Available Since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pBMN-flag beta-catenin K19R/K49R

    Plasmid

    #44751

    Depositor

    Eric Fearon

    Article

    Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

    Insert

    beta-catenin
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K19R/K49R

    Promoter

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

    Plasmid

    #49063

    Purpose

    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid

    #124299

    Purpose

    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    UBE2G1 (UBE2G1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plenti-UbcP-FLAG-YWAA-pGK-HYG

    Plasmid

    #107375

    Purpose

    Lentiviral expression of FLAG-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120393

    Purpose

    pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Diras2 shRNA (Diras2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-3xFLAG-Puro PAK5 K478R K469R

    Plasmid

    #167180

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R K469R
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

    Plasmid

    #51249

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 980,1006 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

    Plasmid

    #133788

    Purpose

    CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized St3Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-mRIPK3 D161N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73705

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    D161N

    Promoter

    CMV/tet

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only