We narrowed to 17,013 results for: Por

1. hSyn-Aion-ts-citrine-ER

   Plasmid

   #192575

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   Aion
   

   Use

   AAV

   Tags

   ts-Citrine-ER

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBF_HsPepT1

   Plasmid

   #73943

   Purpose

   Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

   Depositor

   Simon Newstead

   Article

   Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

   Insert

   PepT1 (SLC15A1 Human)
   

   Use

   Make rna for xenopus oocyte injection

   Tags

   Flag

   Promoter

   beta globin

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pBF_HsPepT2

   Plasmid

   #73944

   Purpose

   Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

   Depositor

   Simon Newstead

   Article

   Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

   Insert

   PepT2 (SLC15A2 Human)
   

   Use

   Make rna for xenopus oocyte injection

   Tags

   Flag

   Promoter

   beta globin

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTAMAHISTEV_PfeAQ482A

   Plasmid

   #128945

   Purpose

   expresses Q482A mutant of PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75), …

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ESR1-Tol2

   Plasmid

   #113382

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ESR1-enhancer (ESR1 Human)
   

   Use

   Tol2 reporter

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-Ef1a-DIO-VGAT-WPRE-pA

   Plasmid

   #39320

   Depositor

   Bernardo Sabatini

   Article

   Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV; R

   Available Since

   Oct. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGAPDH::H2B-mScarlet

   Plasmid

   #186764

   Purpose

   A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   H2B-mScarlet
   

   Use

   Overexpression

   Tags

   H2B

   Promoter

   GAPDH

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP9A-Tol2

   Plasmid

   #113385

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ATP9A-enhancer (ATP9A Human)
   

   Use

   Tol2 reporter

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ5080_pHR_PGK_sfGFP_CoV-F2

    Plasmid

    #155304

    Purpose

    SARS-CoV-2 fluorescent reporter 2

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2 (N, ORF1ab Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. cytosolic moPrP (3F4)

    Plasmid

    #1321

    Depositor

    Susan Lindquist

    Article

    Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

    Insert

    PrP (Prnp Mouse)
    

    Expression

    Mammalian

    Mutation

    N-term truncation (to make gene cytosolic), vario…

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pENeGFP PCNAL2 pc653

    Plasmid

    #167564

    Purpose

    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

    Depositor

    Cristina Cardoso

    Article

    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

    Plasmid

    #134612

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 375 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLS2-YFPc

    Plasmid

    #87166

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Tags

    YFPc

    Promoter

    35s

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. QSOX1-Tol2

    Plasmid

    #113383

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Tol2 reporter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

16. PDZb-beta2(616-951)-mCherry

    Plasmid

    #100736

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Tags

    PDZb and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN_TT 3xFLAG-KPNA2

    Plasmid

    #192306

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNA2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA2 (KPNA2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-IFT27

    Plasmid

    #218722

    Purpose

    Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT27 (IFT27 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLS2-YFPn

    Plasmid

    #87165

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Tags

    YFPn

    Promoter

    35s

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCK314

    Plasmid

    #110545

    Purpose

    Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    Modified PrhaBAD (CRP-binding sites removed)
    yfp
    rhaS
    

    Expression

    Bacterial

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. FastLightR-bRaf-mVenus

    Plasmid

    #162155

    Purpose

    Expresses FastLightR-bRaf-mVenus in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    FastLightR-bRaf-mVenus
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Valine 600 is substituted with Glutamic acid

    Promoter

    CMV

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92228

    Purpose

    Expression of zmADH using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-soCoChR-GFP

    Plasmid

    #107710

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330-sgRNA_Ago2_1

    Plasmid

    #73529

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

28. pX330-sgRNA_Ago2_2

    Plasmid

    #73530

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

29. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1K245 GLUT1-GFP

    Plasmid

    #200097

    Purpose

    human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 6, 7, 225, 229,…

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mcherry Gamma 9 ---pcDNA3.1

    Plasmid

    #64203

    Purpose

    G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    G protein Gamma 9 (GNGT2 Human)
    

    Tags

    6xHis and mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pSpCas9-Puro HLAG-2A-sgRNA

    Plasmid

    #182551

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2A-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

    Plasmid

    #90014

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-TNRC6C
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. shFGF2#2

    Plasmid

    #157668

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPN062

    Plasmid

    #91595

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPN063

    Plasmid

    #91596

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET24a-VHH-tev-std

    Plasmid

    #109423

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ALARM NMR

    Plasmid

    #90209

    Purpose

    Expresses the human La antigen for use in ALARM NMR

    Depositor

    Michael Walters

    Article

    Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

    Insert

    La antigen (SSB Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    contains amino acids 100-324

    Promoter

    T7 promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Promoter

    U6

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge