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We narrowed to 18,759 results for: nls

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Showing: 7681 - 7720 of 18759 results

  1. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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  4. A3G-BE5.13

    Plasmid
    #163637
    Purpose
    C to T DNA base editing
    Depositor
    Xue Gao
    Publication
    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    A3G-BE5.13
    Tags
    BPSV40 NLS
    Expression
    Mammalian
    Mutation
    See manuscript
    Promoter
    CMV
    Available since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    163637-plasmid-map-sequence-id-320316

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  8. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  10. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Publication
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  11. pCAS9c

    Plasmid
    #141250
    Purpose
    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Publication
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141250-plasmid-map-sequence-id-308875

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  17. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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  20. pGL2399

    Plasmid
    #137051
    Purpose
    Expression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCre
    Depositor
    Jeremy Mottram
    Publication
    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi
    Inserts
    Split Cre Recombinase Rapamycin Inducible Dimerisation
    Blasticidin-S Deaminase
    Use
    Cre/Lox; Leishmania ribosomal locus expression
    Promoter
    For integration into the 18S ribosomal locus
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137051-plasmid-map-sequence-id-275797

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  23. AALN-BE4max

    Plasmid
    #138161
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138161-plasmid-map-sequence-id-272350

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  29. pCS2+PhyBΔCreC

    Plasmid
    #131781
    Purpose
    Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.
    Depositor
    George Eisenhoffer
    Publication
    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PhyBCreC
    Use
    Cre/Lox; Zebrafish expression
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    131781-plasmid-map-sequence-id-268075

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  36. pAC1750

    Plasmid
    #127984
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127984-plasmid-map-sequence-id-254510

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  40. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Publication
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122853-plasmid-map-sequence-id-238877

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Showing: 7681 - 7720 of 18759 results