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Showing: 9601 - 9650 of 167210 results

  4. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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  6. pInt_attP1_sacB_kanR

    Plasmid
    #205301
    Purpose
    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205301-plasmid-map-sequence-id-404791

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  9. Pet28a_Synthetic_Human_H2A.1

    Plasmid
    #42634
    Depositor
    Joe Landry
    Insert
    Human H2A.1 (H2AC13 Human, Synthetic)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42634-plasmid-map-sequence-id-57749

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  10. pEGFP-C1-TULP3

    Plasmid
    #218741
    Purpose
    Expresses C-terminally EGFP-tagged TULP3 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    TULP3 (TULP3 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218741-plasmid-map-sequence-id-434240

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  11. RAB27B

    Plasmid
    #25545
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB27B (RAB27B Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. R777-E180 Hs.PIK3R5-nostop

    Plasmid
    #70464
    Purpose
    Gateway ORF clone of human PIK3R5 [NM_014308.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R5 (PIK3R5 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70464-plasmid-map-sequence-id-125913

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  14. pmr101A-cPr-mCherry (ErmR)

    Plasmid
    #177206
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177206-plasmid-map-sequence-id-348606

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  15. mEos2-Moesin-N-14

    Plasmid
    #57402
    Purpose
    Localization: Cytoskeleton/Membrane, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Moesin (MSN Human)
    Tags
    mEos2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. R777-E172 Hs.PIK3R1-nostop

    Plasmid
    #70456
    Purpose
    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R1 (PIK3R1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70456-plasmid-map-sequence-id-125905

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  17. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

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  18. pcDNA3.1-Clover-mRuby2

    Plasmid
    #49089
    Purpose
    Clover-mRuby2 tandem fusion for FRET calibration
    Depositor
    Kurt Beam
    Insert
    Clover-mRuby2 fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    49089-plasmid-map-sequence-id-114654

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  20. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  21. SPLICSFAST-LDh-MT-P2A

    Plasmid
    #214896
    Purpose
    Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214896-plasmid-map-sequence-id-423482

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  22. SPOCK2_22-424_AviN_HisC

    Plasmid
    #195877
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK2 (SPOCK2 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Promoter
    Polyhedrin
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195877-plasmid-map-sequence-id-387267

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  23. pNBU2_repA1

    Plasmid
    #211910
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211910-plasmid-map-sequence-id-421620

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  24. CX3CR1-EGFP

    Plasmid
    #24061
    Depositor
    Dan Littman
    Insert
    CX3CR1 (Cx3cr1 Mouse)
    Use
    Mouse Targeting
    Tags
    EGFP
    Available Since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24061-plasmid-map-sequence-id-483748

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  25. pCAG-N2cG

    Plasmid
    #100811
    Purpose
    express rabies virus CVS N2c strain glycoprotein gene (N2cG)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain glycoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    100811-plasmid-map-sequence-id-189508

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  26. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  27. mCherry-Vinculin-23

    Plasmid
    #55159
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55159-plasmid-map-sequence-id-220874

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  28. YEpADE1

    Plasmid
    #22495
    Depositor
    Beth Montelone
    Insert
    ADE1 (ADE1 Budding Yeast)
    Use
    Yeast shuttle
    Mutation
    Original 4067 bp HindIII-HindIII insertion (see Y…
    Available Since
    Jan. 20, 2010
    Availability
    Academic Institutions and Nonprofits only
    22495-plasmid-map-sequence-id-10339

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  29. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-466943

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  31. pET-C

    Plasmid
    #213496
    Purpose
    Encodes Node C of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    lambda cI 5G6GP
    mCherry
    Use
    Synthetic Biology
    Tags
    ASV tag and LVA tag
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    213496-plasmid-map-sequence-id-430629

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  33. pTCF48 HLA-A*11:01

    Plasmid
    #180449
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*11:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180449-plasmid-map-sequence-id-355113

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  34. pmCherry-Paxillin

    Plasmid
    #80029
    Purpose
    Mammalian expression of mouse Paxillin
    Depositor
    Chinten James Lim
    Insert
    Paxillin (Pxn Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    80029-plasmid-map-sequence-id-365712

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  35. PB CAG-sfGFP-T2A-iCreERT2

    Plasmid
    #177309
    Purpose
    CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Expression
    Mammalian
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177309-plasmid-map-sequence-id-370482

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  36. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  37. pTCF236 HLA-B*40:01

    Plasmid
    #180463
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*40:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180463-plasmid-map-sequence-id-355082

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  39. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

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  42. sfGFP-Peroxisomes-2

    Plasmid
    #54601
    Purpose
    Localization: Peroxisomes, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    sfGFP-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54601-plasmid-map-sequence-id-267741

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  44. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  45. pLenti6MFV SLC25A4

    Plasmid
    #194133
    Purpose
    Expresses ANT1 (SLC25A4) protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    SLC25A4 (SLC25A4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194133-plasmid-map-sequence-id-380659

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  46. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  47. pLenti6MFV SLC40A1

    Plasmid
    #194132
    Purpose
    Expresses MTP1 (SLC40A1) protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    SLC40A1 (SLC40A1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194132-plasmid-map-sequence-id-380658

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  48. RARS_pNIC-Bio3

    Plasmid
    #153055
    Purpose
    Expression construct for producing Avi tagged Arginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    RARS (RARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153055-plasmid-map-sequence-id-304475

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  49. mFGF2

    Plasmid
    #22084
    Depositor
    Gail Martin
    Insert
    FGF2 (Fgf2 Mouse)
    Use
    Bacterial
    Available Since
    Sept. 17, 2009
    Availability
    Academic Institutions and Nonprofits only

  50. pCEV-G2-Km ymNeongreen

    Plasmid
    #193959
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast
    Depositor
    Bas Teusink
    Insert
    ymNeonGreen
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193959-plasmid-map-sequence-id-383894

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Showing: 9601 - 9650 of 167210 results